. dan bai zhi zu xue cong xu lie dao gong neng. botany. 48 èç½è´¨ç»å-¦. kDa .94 â 67 â¢43 -30 â 20 -14 å¾é¼ èæ碱æ§èç½ï¼TCA/ä¸é ®æ²æ·ï¼çåæå IPG-DALT (Gorg et al â¢, 1997) ä¸ç»´ï¼IPG9-12 (5000Væ大çµå8h)ãå离è·ç¦»18cmãå¨é³æ端 ç¨å æ ·æ¯ä¸æ ·ãäºç»´ï¼åç´SDS-PAGE (13%Tè¿ç»-ï¼ãé¶æã 稿ï¼çè¯å¥½ç2-DEå¾è°±ãæ-¤å¤ï¼å¨pH9åpH12é´å«ä¸ä¸ªå¹³ç¼åºçpH4ã12 IPGå¯ä»¥ 对å¦æ ¸ç³ä½èç½è´¨ç±»å¼ºç¢±æ§èç½è´¨è·å¾æ好å离(Gt>rg et al. ï¼ 1998)ãè¿äºå®½æ¢¯åº¦å¯ 以å¨ä¸å«å¼ä¸éçæ åæ¡ä»¶ä¸è¿è¡ï¼å 为æ-¤æ¶è¶ è¿pHlOççªIPGè¶ä¸-æ°´å离å-åé´æ ç强ç转移ï¼ååçµæ¸ï¼ç¹æ§å¯ä»¥å¿½ç¥ä¸è®¡ãæ-¤å¤ï¼é«åå-è´¨éèç½è´¨çæ¸å ¥åèç¦ä¹ å¾å°äºæ
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