. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B 200 150 Q. I •5 50 0> T. funebralis - sun-exposed 7" funebralis - field 200 -, 150 100 50 - 7. brunnea - sun-exposed T. funebralis - sun-exposed. D 03-Apr-OO 10-Apr-OO 17-Apr-OO 24-Apr-OO 01-May-OO Time (days) Figure 2. Environmental variation and time course of heat-shock protein expression (Hsp74) in experimen- tal and control snails. (A) Tide heights, day and night cycles, and mid-intertidal temperatures (see Fig. 1 legend for details). (B) Time course of endogenous levels of Hsp74 for specimens of Tegiila brunn
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B 200 150 Q. I •5 50 0> T. funebralis - sun-exposed 7" funebralis - field 200 -, 150 100 50 - 7. brunnea - sun-exposed T. funebralis - sun-exposed. D 03-Apr-OO 10-Apr-OO 17-Apr-OO 24-Apr-OO 01-May-OO Time (days) Figure 2. Environmental variation and time course of heat-shock protein expression (Hsp74) in experimen- tal and control snails. (A) Tide heights, day and night cycles, and mid-intertidal temperatures (see Fig. 1 legend for details). (B) Time course of endogenous levels of Hsp74 for specimens of Tegiila brunnea transplanted to unshaded (sun-exposed) and shaded mid-intertidal cages versus field-acclimatized conspecifics (shallow subtidal zone). (C) Hsp74 levels for restricted (caged) and unrestricted (field-acclimatized) individuals of Tegula funebralis (mid-intertidal zone) and (D) for T. funebralis and T. brunnea transplanted into unshaded mid- intertidal cages (see Fig. 1 legend for details). stress, there have been few tests of this hypothesis under natural conditions. Furthermore, the ecological importance of interspecific variation in the heat-shock response deduced from laboratory- studies has not been tested in the field. In this study we show that the subtidal to low-intertidal Tegula brunnea transplanted above its natural zone of occurrence experienced sublethal thermal stress, as reflected by ele- vated levels of two isoforms of the 70-kDa family of heat- shock proteins (Hsp72 and Hsp74). In contrast, levels of Hsp72 and Hsp74 varied little in control specimens of Tegula brunnea collected from the shallow subtidal zone, and less in T. brunnea individuals transplanted to shaded mid-intertidal cages. In addition, T. funebralis transplanted within its natural zone of occurrence, to mid-intertidal cages, but not field-acclimatized individuals, showed slightly elevated Hsp levels during a prolonged midday. Please note that these images are extracted from scanned page images
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
