. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 6. Time-course of microtuhule redistribution during clot formation by lipopolysaccharide-activated amebocytes, observed by anti-tubulin immunofluorescence (phase contrast-fluorescence pairs: time of fixation is in upper left corner of phase images a-d). (a, a') Cells 30-s post-activation; MBs are still at the periphery, somewhat folded and buckled, but still recognizable ( a', arrow); most granules have already exocytosed, and boundaries of individual cells are beginning to be obscured by the clot as viewed in ph
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 6. Time-course of microtuhule redistribution during clot formation by lipopolysaccharide-activated amebocytes, observed by anti-tubulin immunofluorescence (phase contrast-fluorescence pairs: time of fixation is in upper left corner of phase images a-d). (a, a') Cells 30-s post-activation; MBs are still at the periphery, somewhat folded and buckled, but still recognizable ( a', arrow); most granules have already exocytosed, and boundaries of individual cells are beginning to be obscured by the clot as viewed in phase contrast (a), ib. b') 3 min after activation; microtubules have moved into the interior and are associated with nuclei; many MBs are still recognizable as highly twisted forms (, arrow), (c. c') 7 min post-activation; MBs are essentially unrecognizable as such, but microtubule bundles are still localized at nuclear surfaces (n; arrow in c'). (d, d'l 10 min post-activation; microtuhules have a much more diffuse distribution, even in the few remaining cells in which exocytosis is incomplete (d, arrow). Bar = 10 | Figure 7. Activation tune-course for F-actin redistribution relative to microtubules during clot formation, observed by anti-tubulin and rhodamine- phalloidin fluorescence (phase contrast-fluorescence triplets); time of fixation is in upper left corner of phase micrographs (a-d). Individual cells are not discernible in phase contrast post-activation, with the image mottled by the surrounding clot, (a-a") I = 1 min; exocytotic cells within the clot have become more compact, with marginal bands (MBs) beginning to deform and twist (a', arrow) and cytoplasmic F-actin distribution in a variable phase (a"), (b-b") t = 5 min; MBs are twisted and located in the interior, closer to nuclei (b' I; spiky F-actin-rich protrusions appear on most cells (b". arrow), (c-c") I = min; MBs have lost recognizability. and microtubule bundles remain in the
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
