. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MICROPROBE MAPS OF SQUID STATOLITHS 129 forms an angle with the lateral dome (plane 3 in Fig. 2). and ten statoliths were broken above the nucleus (plane 1 in Fig. 2). The surface of the break was then viewed under a stereomicroscope, and only statoliths with rela- tively clean and even surfaces were accepted for further preparation. The statolith halves were mounted on a Formvar membrane spread on a thin aluminum frame using Araldite Rapid glue. A second frame with Formvar was glued on top of the statolith's frontal surface
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MICROPROBE MAPS OF SQUID STATOLITHS 129 forms an angle with the lateral dome (plane 3 in Fig. 2). and ten statoliths were broken above the nucleus (plane 1 in Fig. 2). The surface of the break was then viewed under a stereomicroscope, and only statoliths with rela- tively clean and even surfaces were accepted for further preparation. The statolith halves were mounted on a Formvar membrane spread on a thin aluminum frame using Araldite Rapid glue. A second frame with Formvar was glued on top of the statolith's frontal surface and coated with a thin layer of carbon. Once the nuclear mi- croprobe observations had been completed, the samples were re-mounted on aluminum stubs, re-coated with car- bon, and viewed under a Stereoscan 200 scanning electron microscope (SEM). To locate areas of the most prominent increments, statoliths were prepared according to Lipinski and Durholtz (1994) and viewed and photographed under a Zeiss light microscope. Terminology used is after Clarke (1978) and Lipinski et al. (19911. Microprobe measurements The selection of the microanalytical technique for this study was based on two criteria, namely lateral resolution and sensitivity. Ideally, a technique with a lateral resolu- tion of about /jm or less (the spatial scale of incre- ments in squid statoliths). with a sensitivity permitting quantitative mapping of the elements of interest, is re- quired. Since Sr concentration was of particular interest in this study, the analytical technique had to be capable of measuring this element at levels approaching a few parts per million. Although the proton microprobe is capa- ble of this level of sensitivity, it cannot fulfill the criterion for lateral resolution. Techniques that provide such high levels of resolution (such as electron microprobes in en- ergy dispersed spectrometry mode, or electron energy loss spectroscopy) require very thin samples: these are almost impossib
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
