. Laboratory work in bacteriology. Bacteriology. 266 BACTERIOLOGY. obtained by this procedure are more rapid and are far more dangerous than a subcutaneous injection. The injection is made by means of the syringe or injec- tion apparatus, described above. Special care must be ^ taken to see that all air-bubbles are expelled from the syringe before it is used. Air in itself is not dangerous, provided it is not injected rapidly and in large bubbles. It is well, therefore, to begin the injection as slowly as possible. The rabbit is the animal employed most often for this method of injection. This


. Laboratory work in bacteriology. Bacteriology. 266 BACTERIOLOGY. obtained by this procedure are more rapid and are far more dangerous than a subcutaneous injection. The injection is made by means of the syringe or injec- tion apparatus, described above. Special care must be ^ taken to see that all air-bubbles are expelled from the syringe before it is used. Air in itself is not dangerous, provided it is not injected rapidly and in large bubbles. It is well, therefore, to begin the injection as slowly as possible. The rabbit is the animal employed most often for this method of injection. This is due to the fact that the mar- ginal branch of the posterior auricular vein is easily entered with the needle. With a little Fig. 44. The voges cylindrical holder, practice the Operation Can be for guinea-pigs. t,- i done almost as expeditiously as an ordinary subcutaneous injection. It is well to select an animal with white ears, and in which the marginal vein is fairly large. Occasionally this vein is very narrow and it is then extremely difficult to enter. It is advisable, more- over, to use a sharp new needle. The animal is placed on the table before a window. The assistant holds the animal down g'ently but firmly. One hand rests over the pel- vis, the other covers the head and holds the front legs. There is really but one vein in the rabbit's ear which can be used for the pur- pose of injection. The large, middle veins of the ear are imbedded in loose connective tissue, and hence, readily roll away from the needle. The posterior marginal vein is narrow, but it is imbedded in such a manner that it cannot move away. Hence, little difficulty is experi- enced in penetrating this vein although it may seem to be narrower than the needle. By means of a pair of scissors, preferably bent, the hair is re- moved from the surface over this marginal vein. A piece of Please note that these images are extracted from scanned page images that may have been digitally enhanced fo


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