. Biological structure and function; proceedings. Biochemistry; Cytology. DINITROPHENYLATION OF RIBONUCLEASE A 49 detected. At present, however, it is evident that the presence of inhibitor serves to slow the reaction down at relatively few of the available functional groups on the protein molecule. Examination of the values in Table I suggested that the inactivation reaction was primarily due to substitution at an e-amino group. Ray and Koshland have recently developed a treatment for dealing with the results. Fig. I. Kinetics of inactivation of ribonuclease A by dinitrophenylation. An initia
. Biological structure and function; proceedings. Biochemistry; Cytology. DINITROPHENYLATION OF RIBONUCLEASE A 49 detected. At present, however, it is evident that the presence of inhibitor serves to slow the reaction down at relatively few of the available functional groups on the protein molecule. Examination of the values in Table I suggested that the inactivation reaction was primarily due to substitution at an e-amino group. Ray and Koshland have recently developed a treatment for dealing with the results. Fig. I. Kinetics of inactivation of ribonuclease A by dinitrophenylation. An initial lag phase in the reaction is not shown. The conditions of dinitrophenylation were the same as those described in Table I. The points represent the average of two determinations at different concentrations of the remaining ribonuclease activity. The procedure of Gundlach et ol. [8] was used with cyclic cytidylic acid as the substrate. obtained by kinetic analysis of modification reactions with proteins [lo]. With this approach, and on the assumption that during the initial stages of dinitrophenylation (up to 40 minutes in Table I) the drop in the total lysine value is due exclusively to reaction of fluorodinitrobenzene at but two e-amino groups, a pseudo first-order reaction rate constant for these two groups of o-DIG min.~^ was calculated. Determination of the remaining ribonuclease activity in the reaction products during the same experiment. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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