. Biochemical evolution in the slimy salamanders of the Plethodon glutinosus Complex in the Eastern United States. Slimy salamander; Molecular evolution; Plethodon; Amphibians. Maha, Maxson, and Highton: Geographic Variation in Plasma Albumins 91 or d and e of P. cylindraceus (Tables 8 & 9). This inability to discriminate between alleles is not surprising. An antiserum to each allele in the study as well as many more populations would probably be needed to discriminate between all of the different albumins. glu/inosus(c) teyahalee Chattahoochee (d) ctilore6ryontt(b\. chlorobryon, variolatu
. Biochemical evolution in the slimy salamanders of the Plethodon glutinosus Complex in the Eastern United States. Slimy salamander; Molecular evolution; Plethodon; Amphibians. Maha, Maxson, and Highton: Geographic Variation in Plasma Albumins 91 or d and e of P. cylindraceus (Tables 8 & 9). This inability to discriminate between alleles is not surprising. An antiserum to each allele in the study as well as many more populations would probably be needed to discriminate between all of the different albumins. glu/inosus(c) teyahalee Chattahoochee (d) ctilore6ryontt(b\. chlorobryon, variolatus (d)' m/ss/ss/'pp/ id) / glufinosui (d) aureo/usie) ? kentucki (b) ocmulgee (h) Fig. 19. Network of inferred relationships among species in the P. glutinosus complex, indicating all of the common alleles detected by gel electrophoresis. The distances along the branches are immunological distance units. An analysis of the correlation of immunological distance and Nei genetic distance was performed because there are 431 comparisons. The correlation coefficient was , considerably lower than that observed in earlier studies where correlations of between and were reported (Maxson and Maxson, 1979; Highton and Larson, 1979; Wyles and Gorman, 1980). However, all earlier studies involved IDs ranging from 0-80 ID, with very few IDs less than 10 units. This study, on the other hand, involved IDs all less than 14. Despite the small range of immunological distance in these comparisons, the correlation was still significant at the level. The slope of the linear regression line for these data is , also somewhat lower than that in previous studies of salamanders: Hydromantes (28), Pseudoeurycea (23), and Plethodon (24) (Maxson and Maxson, 1979). This variation in slope should serve as a cautionary warning to investigators who use Nei genetic distances to estimate time of divergence from albumin immunological studies. The apparent variation in the slope appears to be att
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