. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LYTIC KI-TKCTS OF SPKRM EXTRACTS Fl. ATK 1 m-m. Effects of Myfilus cdulis sperm extracts on the egg membrane and intercellular cement: a, membranes rendered visible by plasmolysis of eggs with hypertonic sea water; b, dissolution of membranes after two minutes treatment with a sperm extract; c, normal appearance of eggs after first cleavage ; d. cleavage of eggs in a sperm extract. for as long as three or four days. Viable eggs and sperm were obtained by allowing the animals to spawn in individual finger bowls of sea water a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LYTIC KI-TKCTS OF SPKRM EXTRACTS Fl. ATK 1 m-m. Effects of Myfilus cdulis sperm extracts on the egg membrane and intercellular cement: a, membranes rendered visible by plasmolysis of eggs with hypertonic sea water; b, dissolution of membranes after two minutes treatment with a sperm extract; c, normal appearance of eggs after first cleavage ; d. cleavage of eggs in a sperm extract. for as long as three or four days. Viable eggs and sperm were obtained by allowing the animals to spawn in individual finger bowls of sea water at room temperature. Sperm suspensions, used for the preparation of sperm extracts, were obtained either by concentrating naturally spawned sperm by centrifugation or by the shed- ding of sperm from a number of excised testes in a small volume of sea water. The latter procedure proved to be particularly convenient for obtaining very dense sus- pensions of sperm. Sperm extracts were prepared by freezing a sperm suspension followed by thawing and grinding the frozen mass in a mortar. This resulted in a viscous liquid which on centrifugation separated into a white precipitate and a straw colored supernatant. The latter, representing a crude sperm extract, contained the egg membrane lysin and cement dissolving factor. Ripe eggs of M. cdiilis, as obtained by spawning, are about 63 microns in diame- ter, somewhat opaque, and irregularly shaped. There is no egg coat or jelly layer. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
