. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 162 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS Reference: Biol. Bull. 199: 162-163. (October 2000) The Spatial Representation of Odors by Olfactory Receptor Neuron Input to the Olfactory Bulb is Concentration Invariant Matt Wachowiak, Michal Zochowski, Lawrence B. Cohen, and Chun X. Falk {Department of Cellular ami Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520) We wish to understand how odorants are distinguished and how one odorant is recognized as the same across a concentrati
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 162 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS Reference: Biol. Bull. 199: 162-163. (October 2000) The Spatial Representation of Odors by Olfactory Receptor Neuron Input to the Olfactory Bulb is Concentration Invariant Matt Wachowiak, Michal Zochowski, Lawrence B. Cohen, and Chun X. Falk {Department of Cellular ami Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520) We wish to understand how odorants are distinguished and how one odorant is recognized as the same across a concentration range of several orders of magnitude. To this end we have measured the spatial pattern of the olfactory receptor neuron input to the olfac- tory bulb in the three-toed box turtle (Terepene triunguis). To monitor the input to the bulb we labeled the nerve terminals of the olfactory receptor neurons with Calcium Green-1 dextran 10 kD (Molecular Probes) following the method developed by Friedrich and Korsching (1). We then formed a magnified (4X) image of the bulb on an 80 X 80 CCD camera (NeuroCCD; RedShirtlmaging. LLC, Fairfield. CT) and recorded the changes in fluorescence that resulted from a 2-s odorant pulse delivered to the nose. The signals we measured had approximately the same time- course everywhere in the bulb, and we therefore characterized the response by the amplitude of the signal as a function of its position on the bulb. Figure 1 shows three pseudocolor representations of activity in response to the odorant, hexanone. Red represents a large signal in each measurement and blue represents a signal 30% as large. The left-hand image shows the response to hexanone at a concentration that was of saturation. The largest signal in the response was colored red (normalized scaling). Both right-hand images show the Concentration-dependence: normalized vs. absolute maps 10% hexanone normalized scaling — % dF/F hexanone normalized scaling % dF/F
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
