. Manual of bacteriology. Bacteriology. 46 METHODS OF CULTIVATION OF BACTERIA. Streptococci, but Marmorek has succeeded in doing so by grow- ing them on the following media, which are arranged in the order of their utility : — 1. Human serum 2 parts, bouillon i part. 2. Pleuritic or ascitic serum i part, bouillon 2 parts. 3. Asses' or mules' serum 2 parts, bouillon i part. 4. Horse serum 2 parts, bouillon i part. Human serum can be obtained from the blood shed in venesection, the same precautions being taken as in the case of that got in the slaughter-house. In the case of these media, sterili
. Manual of bacteriology. Bacteriology. 46 METHODS OF CULTIVATION OF BACTERIA. Streptococci, but Marmorek has succeeded in doing so by grow- ing them on the following media, which are arranged in the order of their utility : — 1. Human serum 2 parts, bouillon i part. 2. Pleuritic or ascitic serum i part, bouillon 2 parts. 3. Asses' or mules' serum 2 parts, bouillon i part. 4. Horse serum 2 parts, bouillon i part. Human serum can be obtained from the blood shed in venesection, the same precautions being taken as in the case of that got in the slaughter-house. In the case of these media, sterilisation is effected by method B (4), and they are used fluid. Hiss's Serum Media. — Their use is for a more efficient means of differentiating pneumococcus from streptococcus pyo- genes, and they bear some resemblance to those of Marmorek. A. Ox serum i part. Distilled water 2 parts. Normal sodium hydrate o. i per cent. B. Ox serum i part. Distilled water 2 parts. Inulin per cent. They can be sterilised intermittently at 100° C. without coagulating. In either medium pneumococcus forms acid and coagulates the serum, but more rapidly in the latter, whilst streptococcus does neither. Potatoes as Culture Material. (a) In Potato Jars. — The jar consists of a round, shallow, glass vessel with a similar cover {vide Fig. 11). It is washed with i-iooo corrosive sublimate, and a piece of circular filter paper, moistened with the same, is laid in its bottom. On this latter are placed four sterile watch- glasses. Two firm, healthy, small, round potatoes, as free from eyes as Fig. jar. possible, and with the skin whole, are scrubbed well with a brush under the tap and steeped for two or three hours in i-iooo corrosive sublimate. They are steamed in the Koch's steriliser for thirty minutes or. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may no
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