. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. CYCLIC AND NONCYCLIC PHOTO PHOSPHORYLATION 187 S I ^ ^ I TRANSHYDROGENASE ACTIVITY in chromatophores + /imofes DPN. Fig. 2. Transhydrogenase activity in R. rubrio)! chromatophores. The re- action mixture contained, in a final volume of 3 ml, alcohol dehydrogenase, chromatophores (containing 32 ^g bacteriochlorophyll) and the following (in //moles): tris buffer, pH , 100; magnesium chloride, 5; ethanol, 170; TPN, 2. Also present was //moles DPN or extract (see text) as indi- cated. The r
. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. CYCLIC AND NONCYCLIC PHOTO PHOSPHORYLATION 187 S I ^ ^ I TRANSHYDROGENASE ACTIVITY in chromatophores + /imofes DPN. Fig. 2. Transhydrogenase activity in R. rubrio)! chromatophores. The re- action mixture contained, in a final volume of 3 ml, alcohol dehydrogenase, chromatophores (containing 32 ^g bacteriochlorophyll) and the following (in //moles): tris buffer, pH , 100; magnesium chloride, 5; ethanol, 170; TPN, 2. Also present was //moles DPN or extract (see text) as indi- cated. The reaction was carried out in Thunberg type cuvettes at room temperature in the dark. occurred in a complete system in which the photoreduction of DPN was inhibited by the addition of the inhibitor phenyl mercuric acetate (22). Stoichiometry The stoichiometry of the ATP formed and the DPN reduced in the course of noncyclic photophosphorylation is shown in Table 9, The theoretical ratio of ATP/DPNH2 = 1 was obtained when the DPNH2 formed was trapped by an added lactate dehydrogenase system. With- out the lactate dehydrogenase system, the ratio ATP/DPNH2 was. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Symposium on Bacterial Photosynthesis (1963 : Yellow Springs, Ohio); Charles F. Kettering Research Laboratory (Yellow Springs, Ohio); Gest, Howard. Yellow Springs, Ohio, Antioch Press
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