. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REVERSAL OF CELL DIVISION INHIBITION 265 EXPERIMENTAL RESULTS Interference with the hydrogen transport system near its terminus Graphically presented in Figure 1 are the results of typical experiments in which low oxygen tension was used to inhibit cleavage of Arbacia eggs. It is seen that in all instances ATP stimulated cell division of the inhibited eggs. Fur- ther, the added ATP completely reversed the division inhibition provided the latter was not greater than 50 per cent. Figure 2 shows that adenylic acid (AA), ARBACIA
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REVERSAL OF CELL DIVISION INHIBITION 265 EXPERIMENTAL RESULTS Interference with the hydrogen transport system near its terminus Graphically presented in Figure 1 are the results of typical experiments in which low oxygen tension was used to inhibit cleavage of Arbacia eggs. It is seen that in all instances ATP stimulated cell division of the inhibited eggs. Fur- ther, the added ATP completely reversed the division inhibition provided the latter was not greater than 50 per cent. Figure 2 shows that adenylic acid (AA), ARBACIA .O * r6 1 °'5 k ^l | ' ^ <o '§ 024 ATP -i 1 1 1 1- 8 10 12 14 FIGURE 1. The reversal effect of ATP on low oxygen tension-inhibited Arbacia eggs. Rate of oxygen consumption as per cent of control: (1) 75; (2) 60; (3) 50. Experimental duration 100 minutes. hydrolyzed ATP (HATP), or the - P compound, inorganic triphosphate (ITP), were not effective in reversing the division inhibition. Because added ATP completely reversed low oxygen tension inhibition of Arbacia egg cleavage, it was thought a similar reversal might be obtained when cyanide or azide were used as inhibiting agents. The results with sodium azide are presented in Figure 3. The results with sodium cyanide are presented in Figure 4. It is seen that the inhibition of cleavage in Arbacia eggs is: (1) Reversed when ATP is added to cyanide-inhibited cells. (2) Not reversed when ATP is added to azide-inhibited cells. (3) Not reversed when hydrolyzed ATP is added to either cyanide- or azide- inhibited Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947;
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
