. Annals of applied biology. Biology, Economic; Biochemistry. J. Henderson Smith 31 These experiments have been frequently repeated and always with the same general result. In experiments performed on different days and with different spore cultures the minor details never completely correspond, and it is almost impossible at a later date to reproduce exactly the result obtained on a previous occasion. For this many factors are responsible. The age of the culture and the number of spores used are of great importance, as will appear later, but the chief source of the discrepancies hes in the al


. Annals of applied biology. Biology, Economic; Biochemistry. J. Henderson Smith 31 These experiments have been frequently repeated and always with the same general result. In experiments performed on different days and with different spore cultures the minor details never completely correspond, and it is almost impossible at a later date to reproduce exactly the result obtained on a previous occasion. For this many factors are responsible. The age of the culture and the number of spores used are of great importance, as will appear later, but the chief source of the discrepancies hes in the almost insuperable difficulty of obtaining on different days suspensions of spores which are perfectly ahke. Small differences in the degree of moisture in the culture tubes, the tightness or looseness of the cotton wool plugs affecting the oxygen supply, and Survivors 100 «^. 0 Minutes 30 180 FiK. 1. the like, modify the rate and character of the development of the spores, and this is reflected in the mortahty rate. But the general character of the curves remains the same, a sigmoid curve with a stage of in- creasing steepness, a maximum, and finally a stage of decreasing steep- ness, flattening out more and more as time goes on. This is unhke the curve generally accepted as the typical mortahty curve for bacteria exposed to disinfectant agents, such as phenol, cor- rosive subhmate, heat, sunhght, etc. Since the papers of Chick in England (3, 4) and Madsen and Nyman in Denmark (5), the typical mortahty curve has been recognised as a logarithmic one, corresponding in shape to the unimolecular curve, in which the number dying at any. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Association of Applied Biologists; Association of Economic Biologists. [Wellesbourne, Warwick, etc. ] Association of Applied Biolog


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