. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COMPARATIVE SEROLOGY OF CRUSTACEA 279 60 45 II. 1-78 Antl C. bor«&lla ___ 1C. bor*»lls - - - X C. s*pldua _• X M. aquln»do -o-o- X A. alexJundrl. 4 6 8 10 AJTTIOEN DILUTIOH TUBES 12 FIGURE 3. A typical series of precipitin titration curves showing an order of serological relationship among four crabs. The homologous reaction between Cancer borealis and the antiserum produced against it exceeds all others. Then the alignment occurs, in the order of their decreasing curve areas, Callincctes sapidtis, Acanthocarpus
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COMPARATIVE SEROLOGY OF CRUSTACEA 279 60 45 II. 1-78 Antl C. bor«&lla ___ 1C. bor*»lls - - - X C. s*pldua _• X M. aquln»do -o-o- X A. alexJundrl. 4 6 8 10 AJTTIOEN DILUTIOH TUBES 12 FIGURE 3. A typical series of precipitin titration curves showing an order of serological relationship among four crabs. The homologous reaction between Cancer borealis and the antiserum produced against it exceeds all others. Then the alignment occurs, in the order of their decreasing curve areas, Callincctes sapidtis, Acanthocarpus , and Maia squinado, which is in accord with accepted systematic relationships. vestigations are secured. Further, sera frequently have to be collected under field conditions, necessitating the use of preservatives. There are other aspects of the general problem of antigen comparability than those resulting from the chemical action of preservatives which must also be considered before comparisons of ani- mal sera can be made with confidence. Following are the results obtained from attempts to test the affect of some of the variables encountered in the preparation of animal sera for comparative studies in serological systematics. Physical treatment Usual laboratory procedures have been to allow the blood to clot and the sera to be expressed for about 24 hours. The sera are then centrifuged at 2000-3000 for 20 minutes, filtered sterile in Seitz filters, bottled, and stored in the re- frigerator at 3° ± 1 ° C. Any or all of these steps could alter the nature of the serum proteins and thus modify their serological activity. Table III summarizes a series of tests performed to examine the effects of treatment in the laboratory. The sample of blue crab serum designated in the table as Callinectcs sapidus 47-2 repre- sents the sera of 24 large male crabs all bled within one hour. The pooled col- lection of sera was divided into several parts and given various kinds
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
