. Annual report of the Agricultural Experiment Station. Cornell University. Agricultural Experiment Station; Agriculture -- New York (State). Blight Canker of the Apple Tree. 209 4. Blighted fruit of pear (natural infection). 5. Blighted fruit of apple (natural infection). (Two cultures from widely separated local- ities.) 6. Blighted fruit of apple (artificially infected from canker on apple limb). The pure ctiltures of bacteria from these dif- ferent sources were each carried through 12 differentiating kinds of media (see page 210) and a careftd record of growth, characters and reactions mad
. Annual report of the Agricultural Experiment Station. Cornell University. Agricultural Experiment Station; Agriculture -- New York (State). Blight Canker of the Apple Tree. 209 4. Blighted fruit of pear (natural infection). 5. Blighted fruit of apple (natural infection). (Two cultures from widely separated local- ities.) 6. Blighted fruit of apple (artificially infected from canker on apple limb). The pure ctiltures of bacteria from these dif- ferent sources were each carried through 12 differentiating kinds of media (see page 210) and a careftd record of growth, characters and reactions made. The comparative study ^^^- ^4- Bacteria directly ^ . -^ jrom active canker on showed the growth on a given medium to be appJe tree. Stained in the same for all the different cultures, thus carbol fuchsin. Showing various forms oj the or- establishing beyond a doubt the identity of the ganism as they occur in organisms causing the canker on the bodies ^^'^ diseased bark. and limbs of apple and pear trees and the fire- and twig-blight of these trees. A third set of inoculations was made with the bacteria from certain of the above cultures. This was in August and the young shoots of the pear had ceased to grow. The fruits, however, were in just the right stage for infection. The shoots of the apple were still actively growing. Some 10-12 cross inoculations each were made as follows with the bacteria: 1. From canker to twigs of apples. 2. From canker to fruit of pears. 3. From twigs of pears to twigs of apples. All gave nearly 100 per cent of infections. 12. Notes on the Morphology and Cultural Characters of the Organism, Bacillus amylororus (Bur.) DeToni Morphology.— Direct from an active apple tree canker in hanging drop of sterile tap water. Short rods with rounded ends, single in- dividuals nearly oval, long, a little more than half as thick, oc- curring singly, in pairs, fours or even more, end to end. Many but not all motile. The organism was also examined (in hang
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