. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPERM AGGLUTINATION 169 proportionality. Starting with a very strong egg water there is, on dilution, at first very little change in rate then a decrease roughly pro- portional to the dilution. With constant egg water concentrations, de- crease in sperm concentration generally causes the rate to pass through a maximum. In addition, concentrated sperm suspensions may fail to give a visible reaction with certain egg water dilutions that do act on more dilute suspensions. TABLE I Time in seconds for agglutination to become macr
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPERM AGGLUTINATION 169 proportionality. Starting with a very strong egg water there is, on dilution, at first very little change in rate then a decrease roughly pro- portional to the dilution. With constant egg water concentrations, de- crease in sperm concentration generally causes the rate to pass through a maximum. In addition, concentrated sperm suspensions may fail to give a visible reaction with certain egg water dilutions that do act on more dilute suspensions. TABLE I Time in seconds for agglutination to become macroscopically visible on mixing equal volumes of different concentrations of egg water and sperm suspension. Initial egg water = supernatant from a 10 per cent egg suspension, centrifuged after 2 hours. Initial sperm suspension = 30 per cent dry sperm = approx. X 109 spermatozoa per cc. Temperature = 20° C. rev = spontaneous reversal of agglutination within 24 hrs. d = spermatozoa dead within 24 hours. Dilutions of Sperm Suspension Egg Water Dilutions 1 1/2 1/4 1/8 1/16 1/32 1/64 1/128 1 7 6 4 3 3 4 6 (el) 8(d) 1/2 9 7 5 4 4 5 8 14 (d) 1/4 15 8 7 5 5 6 10 17 (d) 1/8 35 14 10 7 7 10 15 20 rev 1/16 105 49 21 14 13 17 28 30 rev rev 1/32 — . — 140 44 35 32 51 62 rev 1/64 — — — 155 190 150 140 110 rev 1/128 — — — — — 420 300 260 These relations are shown in Table I, which contains the data of one of four similar experiments in which varying dilutions of egg water and sperm suspension were employed. As the data show, increasing the egg water concentrations for a given sperm suspension speeds up the reaction, but the increase in rate is greater with the dilute than with the concen- trated egg waters. Decreasing the concentration of sperm for a given egg water at first speeds up the reaction, then retards it. Certain dilu- tions of egg water fail to give a reaction with concentrated sperm but do so with weaker suspensions. Thus l/^2 egg water does not give a visi
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