. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 242 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. mg/ml mg/mll 1mg/ml D 140 - C E lac- _t_ 's! 100 • * 8, c 80 • '> 'U 30 min | 60 • 0) 40 • nr^n i — i U pi > 0 • 1 II 1 n _ 0 1 Antibody concentration (mg/ml) B too- 90- ? 70- c 60- o ~ SO- |g 30 mln| ll 40- | 30- 20 • 10 • o • 075 1 Antibody concentration {mg/ml} C Figure 1. Myosin-II-dependent sliding of actin filament bundles ami vesicle transport in clam oocyte extracts. (A) Successive AVEC-DIC images of a \liding (tain bundle
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 242 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. mg/ml mg/mll 1mg/ml D 140 - C E lac- _t_ 's! 100 • * 8, c 80 • '> 'U 30 min | 60 • 0) 40 • nr^n i — i U pi > 0 • 1 II 1 n _ 0 1 Antibody concentration (mg/ml) B too- 90- ? 70- c 60- o ~ SO- |g 30 mln| ll 40- | 30- 20 • 10 • o • 075 1 Antibody concentration {mg/ml} C Figure 1. Myosin-II-dependent sliding of actin filament bundles ami vesicle transport in clam oocyte extracts. (A) Successive AVEC-DIC images of a \liding (tain bundle in a pseudo-contractile ri}ig. Tlte bundle contains 6 or more actin filaments, the limit of detection of video microscopy (5). The tip of the bundle (asterisk} slides inward the lower left as depicted in each successive image captured at 6 s inten-als. Bar 10 (B) Antibody inhibition experiments. Inhibition of vesicle transport increased as ihe myosin-11-specific antibody concentration increased from to mg/ml. A non-specific rabbit polyclonal (squid myosin-V antibody) was added to the control (labeled 0 mg/ml). (C) Vesicle transport was inhibited by 95% at an antibody concentration of mg/ml. (D) Fluorescence images of the 3-D-actin nenvork at 30 min for each concentration of antibody used. Interactive bundles self-organized into a 3-D nenvork in the control and at ihc low concentrations of antibody. Network formation was blocked at and mg/ml. cytes with a function-blocking myosin-II-specific antibody to in- vestigate the mechanism of movement of myosin-II to the con- tractile ring. Cytoplasmic extracts were prepared from mature oocytes ob- tained from gravid female clams (3, 4). The clarified extracts were diluted 2-fold (protein concentration about 15 mg/ml) and adjusted to pH Nocodazole (50 jam) was added to the extracts to block microtubule assembly, and an ATP regenerating system was added to maintain ATP levels. The final
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
