Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . be onedecimeter in length. The specific rotation, (a)„, may becalculated by means of the following formula, 1 Barfoeds solution is prepared as follows: Dissolve 4 grams of copperacetate in 100 cc. of water and acidify with acetic acid. 12 PHYSIOLOGICAL CHEMISTRY. («)»- ^•/ in which -o = sodium light. a = observed rotation in degrees. p = grams of substance dissolved in I of liquid. / = length of the tube in the specific rotation
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . be onedecimeter in length. The specific rotation, (a)„, may becalculated by means of the following formula, 1 Barfoeds solution is prepared as follows: Dissolve 4 grams of copperacetate in 100 cc. of water and acidify with acetic acid. 12 PHYSIOLOGICAL CHEMISTRY. («)»- ^•/ in which -o = sodium light. a = observed rotation in degrees. p = grams of substance dissolved in I of liquid. / = length of the tube in the specific rotation has been determined and it is desiredto ascertain the per cent of the substance in solution, thismay be obtained by the use of the following formula, The value of p multiplied by 100 will be the percentage of thesubstance in solution. An instrument by means of which the extent of the rota-tion may be determined is called a polariscope or an instrument designed especially for the examinationof sugar solutions is termed a saccharimeter or polarising sac-cliarimeter. The form of polariscope shown in Fig. 3, below, Fig. One Form of Laurent , Microscope for reading the scale; C, a vernier; E, position of theanalyzing Nicol prism; H, polarizing Nicol prism in the tube below thispoint. 13 consists essentially of a long barrel provided with a Nicolprism at either end (Fig. 4. below). The solution under examination is contained in a tube which is placed betweenthese two prisms. At the front end of the instrument is anadjusting eye-piece for focusing and a large recording discwhich registers in degrees and fractions of a degree. Thelight is admitted into the far end of the instrument and ispolarized by passing- through a Nicol prism. This polarizedray then traverses the column of liquid within the tube men-tioned above and if the substance is optically active the planeof the polarized ray is rotated to the right or left. Bodiesro
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