. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 122 LUCY CHERBAS AND PETER CHERBAS Thin layer chromatography of the extracts distinguished five metabolites of *-ecdysone. Figure 4 shows the dramatic changes in the pattern of metabolites with time, as seen in a pupa injected with /*g of «-ecdysone-3H. The metabolite labeled /? migrates with pure /?-ecdysone in this solvent system as well as in cyclohexane-butanol (6:4). The other metabolites resolved in these chromato- grams are designated I, III, and IV. Metabolite IV is found in trace quantities in most extracts, but
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 122 LUCY CHERBAS AND PETER CHERBAS Thin layer chromatography of the extracts distinguished five metabolites of *-ecdysone. Figure 4 shows the dramatic changes in the pattern of metabolites with time, as seen in a pupa injected with /*g of «-ecdysone-3H. The metabolite labeled /? migrates with pure /?-ecdysone in this solvent system as well as in cyclohexane-butanol (6:4). The other metabolites resolved in these chromato- grams are designated I, III, and IV. Metabolite IV is found in trace quantities in most extracts, but is not seen in control extracts such as that in Figure 4a. Metabolite I does not appear after injection of of a-ecdysone; instead, an- other metabolite of slightly different mobility, labeled II, is seen. The mobilities of all these metabolites, as well as those of a number of ecdysone analogs, are shown in Table I. The quantity of each component was determined from graphs such as those in Figure 4. The minor component IV never represented a fraction high enough to be estimated accurately. Figure 5 shows the time course of appearance of the various metabolites after injection of or jug of a-ecdysone-3H. There is an initial lag time; all of the a-ecdysone curves extrapolate to 100% at 1-2 hours after 100 80 60 40 > H > I- O O 40 20 . o J- z UJ o (£ 40 UJ Q. 20 20. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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