. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I 2 FIG. I. Oogonium, stained with iron hsematoxylin, showing " Nebendotter," and nucleus with chromatin reticulum. FIG. 2. Oogonium showing same structures as Fig. I, but from a much later generation. surrounding cytoplasm. Its reactions to various stains deserve metion. With iron haematoxylin it stains readily, appearing as a dark homogeneous mass even after a great deal of extraction of the stain. The nucleus and cytoplasm may be entirely decolor- ized and the "Nebendotter" still show as a dark body, a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I 2 FIG. I. Oogonium, stained with iron hsematoxylin, showing " Nebendotter," and nucleus with chromatin reticulum. FIG. 2. Oogonium showing same structures as Fig. I, but from a much later generation. surrounding cytoplasm. Its reactions to various stains deserve metion. With iron haematoxylin it stains readily, appearing as a dark homogeneous mass even after a great deal of extraction of the stain. The nucleus and cytoplasm may be entirely decolor- ized and the "Nebendotter" still show as a dark body, a fact which led to confusion during the early part of my study, as the nucleus was overlooked and the " nebendotter " taken for it. The true state of affairs was not revealed until I had used another method of staining when the appearances which with iron haema- toxylin had misled me were explained and the structure of the cells became clear. The new reagents were Kernschwarz counter- stained with Litchtgriin. Kernschwarz is a weak stain affecting only the nucleus. In my preparations I have seen no trace of it in the cytoplasm or in the " ; Lichtgrun stains both nuclear plasm and cytoplasmic structures. The result, then, with this method of staining is as follows : chromatin and nuclear. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
