. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 4. RFLP genotypes of mixtures of Symbiodinium E and C. Samples of zooxanthellae are from Montastraea faveolata (samples 1. 2) and M. franksi (sample 3): other lanes are clones E" ' and C" singly (1:0 and 0:1. respectively) and mixed together in molar ratios ranging from 8:1 to 1:8. to obtain standards. srDNAs were amplified with host-excluding PCR primers and then digested with Dpn II (top panel) and with Tmi I (bottom panel). By visual inspection, samples 1-3 contain both Synihin- iliiiiiini E and C. in rat
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 4. RFLP genotypes of mixtures of Symbiodinium E and C. Samples of zooxanthellae are from Montastraea faveolata (samples 1. 2) and M. franksi (sample 3): other lanes are clones E" ' and C" singly (1:0 and 0:1. respectively) and mixed together in molar ratios ranging from 8:1 to 1:8. to obtain standards. srDNAs were amplified with host-excluding PCR primers and then digested with Dpn II (top panel) and with Tmi I (bottom panel). By visual inspection, samples 1-3 contain both Synihin- iliiiiiini E and C. in ratios of about 4:1. 1 and 1:4. respectively. Lane M contains DNA size standards, as in Figure 2. 1998]): separate from Symbiodinium A. B. and C; and close to a zooxanthellar srDNA from the coral Montipora panda, an srDNA that previously could not be assigned to either Syni- hiodiniwn A. B. or C (Rowan and Powers, 1991a). srDNA from Symbiodinium D. a dinoflagellate cultured from the sponge Haliclona koremella (Carlos ct 1999), is not similar to genotype E (Fig. 3). Thus, genotype E represents a distinct taxon of zooxanthella—Syinbiodinium E. Some samples of zooxanthellae (see below) had RFLP genotypes that implied mixtures of Symbiodinium E and C, based on comparisons to RFLP genotypes of synthetic mixtures of cloned genes (srDNA clones E""' and C°; Fig. 4). As with mixtures of Symbiodinium A. B, or C de- scribed previously (Rowan and Knowlton, 1995; Rowan ct ill., 1997). the apparent ratio of Symbiodinium E to Symbiodinium C in different samples varied, and did not depend on which restriction enzyme was used to differ- entiate these two genotypes ( Fig. 4, Dpn II digests versus Taq I digests). Distribution of different taxa of Symbiodinium At Rio Carti, M. franksi was observed with only two taxa of zooxanthellae—Symbiodinium E and C—and the same two taxa were obtained from M. faveolata and M. annularis (Fig. 5) at this reef. Symbiodinium E was the
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