. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PORICHTHYS PHOTOPHORE EXCITATION 17 100 /A) arranged in a chamber as shown in Figure 1. This is a double chamber: part A is a hollow cylinder 1 cm high, 2 cm inner diameter and cm outer diameter, with a 4 mm diameter hole drilled in the base. A thermostated fluid flow's through the space between the inner and outer walls. Part B is a lucite cylinder hollowed out in order to contain part A to a depth of 5 mm. Two grooves in the floor hold the electrodes. When the two parts are fitted together as shown in Figure 1C, the ph
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PORICHTHYS PHOTOPHORE EXCITATION 17 100 /A) arranged in a chamber as shown in Figure 1. This is a double chamber: part A is a hollow cylinder 1 cm high, 2 cm inner diameter and cm outer diameter, with a 4 mm diameter hole drilled in the base. A thermostated fluid flow's through the space between the inner and outer walls. Part B is a lucite cylinder hollowed out in order to contain part A to a depth of 5 mm. Two grooves in the floor hold the electrodes. When the two parts are fitted together as shown in Figure 1C, the photophore protrudes into part A through the hole in its base and tissues surrounding the light organ are pressed between parts A and B, preventing movement of the specimen. 3. Recording light emission A photomultiplier tube (RCA Type 1P21) was covered by a light-tight tube with an opening at the level of the photocathode. A ring was attached to the opening to accept the photophore chamber (Fig. 2). Thus the specimen could be IP 21 Power supply Gas Chart recorder Thermostat FIGURE 2. Diagram of apparatus used in detecting and recording luminescent responses to electrical stimulation. enclosed with the light organ facing the photocathode at a distance of 15 mm. Oxygen, air, or nitrogen was passed into this enclosure through a small needle crossing obliquely the inner and outer walls of part A. Except for the nitrogen, the gases were humidified before reaching the photophore, which was stimulated in the gas phase. Stimulating electrodes contacting the dermal surface of the photophore were connected to a Grass stimulator delivering square pulses. A stimulus artifact was registered by one of the two channels of a Tektronix 502A oscilloscope and one channel of a chart recorder (Offner Dynograph) connected in series to the oscilloscope. The luminescent response from the specimen activated the photo- multiplier tube whose electrical signal was recorded on the second channel of the
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
