. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. H. TRAPIDO-ROSENTHAL ET AL sured by monitoring the conversion of radiolaheled argi- nine to citrulline (8, 10). We examined the effect of elevated temperature on zoo- xanthellar NOS activity (amol citrulline/zooxanthella/min) isolated from the branching scleractinian coral Madracis miruhilis (senxu Wells. 1973). Corals, from the coastal waters of Bermuda collected by scuba diving, were kept in seawater collected at the site and returned to the laboratory within 1 h. For temperature treatments in the laboratory, corals were m
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. H. TRAPIDO-ROSENTHAL ET AL sured by monitoring the conversion of radiolaheled argi- nine to citrulline (8, 10). We examined the effect of elevated temperature on zoo- xanthellar NOS activity (amol citrulline/zooxanthella/min) isolated from the branching scleractinian coral Madracis miruhilis (senxu Wells. 1973). Corals, from the coastal waters of Bermuda collected by scuba diving, were kept in seawater collected at the site and returned to the laboratory within 1 h. For temperature treatments in the laboratory, corals were maintained at ambient temperature in continu- ously flowing seawater for at least 4X h before treatment. Corals were subjected to temperature stress by placing them in beakers of artificial seawater (ASW; 423 mM NaCl. 26 mM MgSO4-7H:O. 23 mM MgCK-6H:O. 9 mM KC1, mM CaCl2-2H2O. mM NaHCO,. 10 mM HEPES; pH ). The beakers were then placed into incubators set at the desired temperatures and remained there at constant tem- perature and irradiance for 24 h. Algal NOS activity was determined for those zooxanthellae retained within the coral host tissue ( in Iwspite) over the course of the incubation and for those zooxanthellae that had left the host as a result of temperature stress. For in Iwspite zooxanthellae. corals were removed from the incubation at selected time points; zooxanthellae were then separated from the host tissue, as described by Owen et tti (11). For determination of algal NOS activity for zooxanthellae that had left the host, algae were quantitatively collected from beakers by centrifuga- tion. In all cases zooxanthellar numbers were determined by counting with a hemacytometer; cells were then rinsed with homogeni/ation buffer (HB; 50 mM HEPES. 1 mMEDTA; pH , ambient temperature) and homogenized in HB. in a total volume of 1 ml. by vortexing with sterile borosilicate glass beads (a single 5-mm bead. g 710-1 180-/im beads, and g 15-212-jum
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
