. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GLUTAMATE IN SEA ANEMONE ECTODERM 51. • „ ra - Figures 3, 4. Glutamate immunoreactivity in nematocysts of a tentacle (Fig. 3) and its absence in another (control) tentacle (Fig. 4) from the same specimen ofMetridittm jen/Ve (wholemounts). E. ectoderm. Figure 3. Density of stained nematocysts diminishes from distal tip toward the base of the tentacle. Figure 4. Tentacle was treated with primary antibody preabsorbed with -y-Glu-Glu; no staining was detected. Scale bar = 100 ^m. of the ectoderm (Fig. 5). Many such processes hav
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GLUTAMATE IN SEA ANEMONE ECTODERM 51. • „ ra - Figures 3, 4. Glutamate immunoreactivity in nematocysts of a tentacle (Fig. 3) and its absence in another (control) tentacle (Fig. 4) from the same specimen ofMetridittm jen/Ve (wholemounts). E. ectoderm. Figure 3. Density of stained nematocysts diminishes from distal tip toward the base of the tentacle. Figure 4. Tentacle was treated with primary antibody preabsorbed with -y-Glu-Glu; no staining was detected. Scale bar = 100 ^m. of the ectoderm (Fig. 5). Many such processes have a twisted or folded appearance (Fig. 5), presumably as a result of buckling in the tentacle. They seem to the layer of muscle feet of the musculoepithelial cells of the longitudinal musculature, associated cell bodies showing no clear evidence of staining (Fig. 5). Staining was present on both oral and aboral sides of the tentacles and usually extended from the base of the tentacles to within a short distance ( mm) of their tip. Ectodermal granule-containing cells The third immunoreactive site was localized in the ec- toderm of the oral disk proximal to the base of the ten- tacles (Fig. 6). The immunoreactivity was associated with pear-shaped cells (9 ^m in perikaryon length) tapering to a single axon-like process and filled with large granules, 1-2 urn in diameter (Figs. 6. 7). There was no clear evi- dence that the granules themselves had stained. The pro- cesses were up to 40 ^m in length and approximately par- allel. The stained granular cells were distributed over the radial muscle in that area. The radial muscle itself was not stained. Discussion Our observations demonstrate the presence of gluta- mate immunoreactivity in the tentacles and oral disk of M. senile, in agreement with Carlyle's (1974) biochemical detection of large amounts of glutamic acid in the oral margin of the sea anemone A. equina. The lack of spec- ificity of induced glutamat
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
