. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EFFECT OF RINGER'S SOLUTION ON TAIL-REGENERA- TION IN THE TADPOLE OF RANA PALUSTRIS LEONARD P. SAYLES (From the College of the City of Nciv York and the Marine Biological Laboratory, Woods Hole, Mass.) It lias been shown that the regenerative activity of the fresh-water oligochaete Liimbricuhis is decreased in balanced salt solutions (Ringer) which are isotonic, or nearly so, with the body fluids of that worm (Sayles, 1928). Also, in the case of certain marine invertebrates, it has been found that dilutions of the sea water
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EFFECT OF RINGER'S SOLUTION ON TAIL-REGENERA- TION IN THE TADPOLE OF RANA PALUSTRIS LEONARD P. SAYLES (From the College of the City of Nciv York and the Marine Biological Laboratory, Woods Hole, Mass.) It lias been shown that the regenerative activity of the fresh-water oligochaete Liimbricuhis is decreased in balanced salt solutions (Ringer) which are isotonic, or nearly so, with the body fluids of that worm (Sayles, 1928). Also, in the case of certain marine invertebrates, it has been found that dilutions of the sea water increase the rate of regenera- tion (Loeb, 1892; Goldfarb, 1914; Lloyd, 1914; Keil, 1932 and others). In the case of marine forms, of course, the body fluids are approximately isotonic with undiluted sea-water. With these facts in mind a study was made to determine whether or not a similar slowing-up of regeneration in isotonic salt solutions might occur in the case of frog tadpoles. Tadpoles of Rana palnstris, varying in total length from 40 to 65 millimeters and with small posterior legs, were used. They were first anaesthetized in a solution of approximately 1 part of chloretone in 2,200 of water. Either 15 or 20 mm. of the tail were then removed. The first experiments on this material were carried out during the summer of 1927. Then, in 1929 and again in 1932, results essentially the same as those of the first year were obtained. The results of two experiments, typical of a number performed, may be presented to indicate the effect of Ringer solutions on the length and form of the bud of new tissue. In one instance, four tadpoles were cut. Tadpole A (52 mm. total length) was placed in water. B (55 mm. long), C (50 mm. long), and D (40 mm. long) were placed in buffered frog Ringer. All four had 15 mm. of tail removed. After two weeks the lengths of the new buds were as follows: A, mm.; B, mm.; C, mm.; D, mm. All of these were killed at the end of the third wee
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
