. Boletin de la Sociedad de Biología de Concepción. Sociedad de Biología de Concepción; Biology; Biology. Bol. Soc. Biol. de Concepción, Chile. Tomo Lili, 1982. 109 In each experiment the same rat served as both test and control in order to elimínate possible individual differences that might arise due to the administration of ethanol. Gr. Fig. Schematic the prepaiation of small intestine of the rat. = saline; = polistaltic pump; = bath at a normal temperature; M = manometer; = small intestine; = prass amplifier; R = recorder; S = samples. Water
. Boletin de la Sociedad de Biología de Concepción. Sociedad de Biología de Concepción; Biology; Biology. Bol. Soc. Biol. de Concepción, Chile. Tomo Lili, 1982. 109 In each experiment the same rat served as both test and control in order to elimínate possible individual differences that might arise due to the administration of ethanol. Gr. Fig. Schematic the prepaiation of small intestine of the rat. = saline; = polistaltic pump; = bath at a normal temperature; M = manometer; = small intestine; = prass amplifier; R = recorder; S = samples. Water absorption was estimated by measuring the volumen differences obtained between the test and control solutions. Sodium and chloride ions were determined electrometrically by the use of ion-specific electrodes (Thomas, model 4923-L 10 sodium and Orion model 92-17 chloride electrodes). Glucose was estimated by means of the o-toluidine procedure (Hyvarinen and Esko, 1962) and tryf>- tophan was determined by the procedure of Laties (Laties, 1949). When the beta-receptor blocker propranolol was used, a final concentration of 1 x 10^'^M was added to the perfúsate after coliecting a control sample. In those experiments in ivhich the effects of norepinephrine were studied, one mi of a 1 x lO'^^M dibenzyline solution in saline was parenterally injected two hours before the infusión of saline containing a final concentration of x lO'^'M norepinephrine or 652 mM ethanol. D-Glucose (5mM) and L-Tryptophan ( mM) were prepared in saline and were perfused in the presence and absence of ethanol. RESULTS Fig. 2 shows that x 10"=M Norepinephrine added to the lu- minal side elicits a statistically significant (p<) increase in the ab- sorption of water, sodium and chloride, with significant changes in P. D. The same results were observed when Norepinephrine was substituted for Ethanol (652 mM) (see Fig. 3).. Please note that these images are extracted from scanned
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