. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. J^ I i#i' b . /- i Figure 6. Early response in the tunic reaction of Ciona intestinalis after erythrocyte injection, (a, b) Transverse sections showing tunic wound (tw) and amoebocyte (a) infiltration, (a) Twelve hours after injection, 160X. (b) Twenty-four hours after injection, 390X. (c) Benzidine-positive amoebocytes, 980X. (d) Hyaline amoebocyte (arrow), 980X. (e) Granular amoebocyte (arrow), 980X. g, granule; v, vacuole, a, b, d, e: Mallory stained. showed lobose pseudopodia. Some vesicular cells lined up forming an enc
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. J^ I i#i' b . /- i Figure 6. Early response in the tunic reaction of Ciona intestinalis after erythrocyte injection, (a, b) Transverse sections showing tunic wound (tw) and amoebocyte (a) infiltration, (a) Twelve hours after injection, 160X. (b) Twenty-four hours after injection, 390X. (c) Benzidine-positive amoebocytes, 980X. (d) Hyaline amoebocyte (arrow), 980X. (e) Granular amoebocyte (arrow), 980X. g, granule; v, vacuole, a, b, d, e: Mallory stained. showed lobose pseudopodia. Some vesicular cells lined up forming an encapsulating strip while others filled the tunic matrix and may be in shape. Large refringent granule cells are numerous inside the wound and are also present in the inner tunic layer below the wound. Nodules of transitional cells are observed near the epithelium. In tunics fixed after 24 hours, the above cells are more numerous. On the wound inner edge, the cells are full of aniline blue-stained material (Fig. 6b) while the eryth- rocyte masses are not easily distinguished. After 48 hours many compartment cells, with vacuoles containing yellow refringent material alone were found inside the wound. Granule-packed cells were also present in the tunic while large vacuoles characterized the epithelial cells. At 12 and 24 hours the large round cells inside the wound and the amoebocytes were positive to the benzidine histochemical reaction for hemoglobin (Fig. 6c). Tests carried out at 48 hours and 6 days were negative. The tunics from specimens which 6-7 days after SE injection did not show an apparent capsule, presented in the injection site the same components found in the early stages (12-48 hours) of the capsule development. Low general cell frequency and few granule-packed cells characterize these tunic areas. The capsule increases in thickness and 12-20 days after 5 X 10^ SE injection, forms a protuberance of the tunic and a decreased number of cells. The wound was re
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