. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SYMBIOTIC PLASTID GENES IN SLUGS 41 CAP. CHX Figure 6. Immunoblot (IB) of LHCI synthesized in the presence of CAP and 35[S]-methionine, and its corresponding autoradiograph (CAP). The arrows indicate radiolabeled bands coinciding to LHCI immunola- beled bands shown in (IB). The bands in (CAP) are not labeled in the presence of CHX (CHX). result seems to indicate that the polypeptides are not LHCII, but since the similarity between the green plant and chro- mophyte LHC proteins is relatively low (Green and Durn- ford, 1996; D
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SYMBIOTIC PLASTID GENES IN SLUGS 41 CAP. CHX Figure 6. Immunoblot (IB) of LHCI synthesized in the presence of CAP and 35[S]-methionine, and its corresponding autoradiograph (CAP). The arrows indicate radiolabeled bands coinciding to LHCI immunola- beled bands shown in (IB). The bands in (CAP) are not labeled in the presence of CHX (CHX). result seems to indicate that the polypeptides are not LHCII, but since the similarity between the green plant and chro- mophyte LHC proteins is relatively low (Green and Durn- ford, 1996; Durnford et al., 1999), we probably cannot completely eliminate the possibility that the anti-LHCI is binding LHCII polypeptides. However, just like LHCI, all of the LHCII genes are nuclear encoded in the plants and algae where they have been found (Jansson, 1994, 1999; Wollman et 1999), and even if we have identified LHCII, the conclusion is still the same: that an algal LHC gene has been transferred to the DNA of the slug. The immunoprecipitations provide additional evidence that the LHCI polypeptides are being synthesized on the cytoplasmic ribosomes in the slug. The high amount of radioactivity precipitated by the antibody in the presence of CAP compared to that precipitated in the presence of CHX demonstrates that the proteins recognized by the anti-LHCI are indeed synthesized in the slugs. Since the amount of radioactivity incorporated varied from slug to slug and from experiment to experiment, we had to normalize the immu- noprecipitation data as percent of control in order to com- pare them. However, in a typical experiment, the values for the amount of radioactive material incorporated into the precipitate in the presence of CAP ranged from 5000 to 25,000 cpm, whereas those in the presence of CHX ran from 150 to 400 cpm, which may give a clearer picture of the level of material bound by the antibody. The results of the pharmacological experiments, the im- munoblots
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