. The microscope; an introduction to microscopic methods and to histology. Microscopes. 190 PARAFFIN SECTIONING [CH. VII 'i 296. Counterstaining with Picro-fuchsin. —For a general dye to use with hematoxylin, eosin is good, but to differentiate the tissues more completely, especially connective tissue, which is present in practically every section made, it is better to use Van Gieson's mixture of picric acid and acid fuchsin. (Picric acid, saturated aqueous solution 75 cc, water 25 cc. !% aqueous solution of acid fuchsin, 10 cc.) Sections are first strongly stained with hematoxylin, well washe
. The microscope; an introduction to microscopic methods and to histology. Microscopes. 190 PARAFFIN SECTIONING [CH. VII 'i 296. Counterstaining with Picro-fuchsin. —For a general dye to use with hematoxylin, eosin is good, but to differentiate the tissues more completely, especially connective tissue, which is present in practically every section made, it is better to use Van Gieson's mixture of picric acid and acid fuchsin. (Picric acid, saturated aqueous solution 75 cc, water 25 cc. !% aqueous solution of acid fuchsin, 10 cc.) Sections are first strongly stained with hematoxylin, well washed with water and then stained 3 seconds to 15 minutes in the picro-fuchsin. They are then washed in distilled water ; or in tap water, to which has been added a drop or two of glacial acetic acid to 100 cc. of water. They are then dehydrated, cleared and mounted in acid balsam, that is in balsam which has not been neutral- ized ($ 315). If glycerin or glj-cerin jelly is used as a mounting medium it should be slightly acid. Unless the mounting medium is slightly acid, the red of the acid fuchsin soon fades. In some cases less acid fuchsin should be used, and in some a greater amount. Acid fuchsin alone without the picric acid is also good for a counterstain. The picro-fuchsin is a very valuable differential stain and combined in different proportions with picric acid will give great assistance in al- most every case. It does not seem to be a permanent stain. (See Freeborn, Trans. X. Y. Path, Soc, 1893, p. 73. Also studies from the department of pathol- ogy of the College of Physicians and Surgeons, Columbia University, N. Y., 1S94-1S95). 161. Coplin's staining dish. A. The entire dish ; B. the dish in cross section. This is made 0/ glass and is a very neat piece of apparatus. With it ten slides may be stained at once. ( Whitall, Tatum & Co.) \ 297. Dehydration of the Stained Sec- tions.—Place the slide with the stained sec- tions in a jar of 95% or absolute alcohol and l
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