. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALPHA AMYLASE IN CRASSOSTREA 201 cr ID o cc UJ CL UJ cc o o o ce UJ a. UJ CO O to o cc o 12 10. 12 18 24 30 HOURS AFTER FERTILIZATION 36 42 FIGURE 1. Alpha amylase activity in homogenates of developing oyster larvae, grown at 28° C. The different symbols represent different batches of eggs. The range of values obtained is shown for each stage. The incubation mixture contained potato starch, 2% NaCl, and M sodium phosphate, pH in the lumen of the midgut. This may represent a larval precursor to the crystal- lin


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALPHA AMYLASE IN CRASSOSTREA 201 cr ID o cc UJ CL UJ cc o o o ce UJ a. UJ CO O to o cc o 12 10. 12 18 24 30 HOURS AFTER FERTILIZATION 36 42 FIGURE 1. Alpha amylase activity in homogenates of developing oyster larvae, grown at 28° C. The different symbols represent different batches of eggs. The range of values obtained is shown for each stage. The incubation mixture contained potato starch, 2% NaCl, and M sodium phosphate, pH in the lumen of the midgut. This may represent a larval precursor to the crystal- line style. Amylase activity in homogenates Data on enzyme activity in homogenates are presented in Figure 1. The enzyme was not detected hefore 17 hours of development, even with homogenate concentrations of 50% in the assay mixtures, and with incubation times up to three hours. The increase in activity between 17 and 37 hours is nearly linear. j Extrapolation of this activity curve shows that the increase probably begins at about 15 hours. After 37 hours a sharp decrease in activitv occurs. The decrease is evidently not a result of the release of the enzyme into the medium, since no amylase activity could be detected in water in which concentrated suspensions of 37-hour embryos had been incubated for two hours. The average values obtained in five determinations of total protein in the homogenates are as follows (X 1O1" grams per egg) : unfertilized eggs. ± ;. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology