Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . n of gaswill be observed at once and will cease in a short time. The carbon dioxidgiven off by the reaction of the urea upon the sodium hypobromite is absorbedby the excess of alkali and the nitrogen collects in the upper portion of thetube. As soon as the evolution of gas has ceased (5 to 10 minutes),the amountof urea is read off directly from the calibrations of the tube as previouslydescribed. In this determination the urine should be free from both albumin andsugar and should cont
Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . n of gaswill be observed at once and will cease in a short time. The carbon dioxidgiven off by the reaction of the urea upon the sodium hypobromite is absorbedby the excess of alkali and the nitrogen collects in the upper portion of thetube. As soon as the evolution of gas has ceased (5 to 10 minutes),the amountof urea is read off directly from the calibrations of the tube as previouslydescribed. In this determination the urine should be free from both albumin andsugar and should contain preferably not more than i per cent, of urea. Itis, therefore, wise to dilute the urine before making the test, although thewriter has seldom observed variations sufficient to lead him to dilute the urinein all cases. The forms of this apparatus which substitute for the glassfoot a wooden base are much to be preferred as they are not so easily modification of this instrument, as introduced by Hinds, is seen in theaccompanying cut. In this form the urine is allowed to run in from the smaller. /^Ocl ^^••^« Fig. 72.—Doremus ureometer. 212 DIAGNOSTIC METHODS. graduated tube by opening the stop-cock. This modification is an advantage,but does not yield any more accurate results than does the preceding. Itis to be repeated that this apparatus is absolutely useless in scientific work,but does furnish a rough and ready method for the use of the general practi-tioner in his daily determination of the urea excretion. Folins^ Method. The principle of this method is asfollows: At a temperature of i6o° C. crys-tallized magnesium chlorid (MgCl26H20)boils in its water of crystallization. If ureabe present it is decomposed by this boilingsolution into ammonia and carbon the conversion be carried out in acidsolution, the ammonia formed will combinewith the acid and may then be liberated byalkalinizing the mixture. The ammonia isdistilled into a standard acid sol
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