. The microscope; an introduction to microscopic methods and to histology. Microscopes. Ch. XI] STAINING MICROSCOPIC OBJECTS 395. Fig. 236. Small Aquaeium Jars for Stain- rNG Serial Sections. R Rack for the top of the jar and contain- ing a small draining funnel. At the left is a spirit lamp used as a balsam bottle. ral waters. One could use distilled water, adding a few drops of a saturated solution of lithium carbonate. Dehydrate in 95% alcohol and absolute if necessary; clear and mount in balsam as described in § 513. Hematoxylin is so nearly a pure nuclear stain for most tissues and organs
. The microscope; an introduction to microscopic methods and to histology. Microscopes. Ch. XI] STAINING MICROSCOPIC OBJECTS 395. Fig. 236. Small Aquaeium Jars for Stain- rNG Serial Sections. R Rack for the top of the jar and contain- ing a small draining funnel. At the left is a spirit lamp used as a balsam bottle. ral waters. One could use distilled water, adding a few drops of a saturated solution of lithium carbonate. Dehydrate in 95% alcohol and absolute if necessary; clear and mount in balsam as described in § 513. Hematoxylin is so nearly a pure nuclear stain for most tissues and organs that the cell bodies are not very evident with this alone; hence some counterstain is gen- erally used also. § 640. Counterstaiiiing with eosin. — One of the solutions of eosin (§ 562) is dropped upon the sec- tions after the hematoxy- lin has been washed away with water. This stains almost instantly. One rarely needs to stain with eosin over 10 or 30 sec- , onds. The excess stain is then washed away with pipette or by dip- ping the slide into water. § 641. Dehydrating and clearing. — Put the slide directly into 95% alcohol after it is rinsed with water. Leave it in the alcohol a short time and transfer to fresh 95 % alcohol or to absolute alcohol a few seconds, 10-20. One must not leave the sections too long in the alcohol or the eosin wilj dissolve out. Remove the slide from the alcohol and put it into a jar of clearer (§ 552) or put it on the rack (fig. 235-236) and add enough clearer to cover the sections. Soon the clearer will displace the alcohol and make the sections translucent. It usually requires only half a minute or so. The clearer is drained off and balsam put on the sections, and then a clean cover-glass is added. One soon learns to use the right amount of balsam. It is better to use too much than too little (§ 513). § 641a. — In the past the plan for changing sections from 95 % alcohol to water, for example, has been to run them down gradually, usin
Size: 2405px × 1039px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, booksubjectmicroscopes, bookyear
