. Pathological technique; a practical manual for workers in pathological histology and bacteriology. , showing the clubs. The preparation not stained; moderately high mag-nifying power (Wright and Brown). margins of which, on close inspection, fine radial striationsor filaments or hyaline club-shaped bodies, all closely settogether, may be seen (Figs. 106, 107). The club-shaped 364 PA THOL OGICAL TECHNIQUE. bodies are variable in size, and are composed of a hyaline,refringent substance. The appearance of radial striation inthe granule, when observed with the microscope, due to thepresence and


. Pathological technique; a practical manual for workers in pathological histology and bacteriology. , showing the clubs. The preparation not stained; moderately high mag-nifying power (Wright and Brown). margins of which, on close inspection, fine radial striationsor filaments or hyaline club-shaped bodies, all closely settogether, may be seen (Figs. 106, 107). The club-shaped 364 PA THOL OGICAL TECHNIQUE. bodies are variable in size, and are composed of a hyaline,refringent substance. The appearance of radial striation inthe granule, when observed with the microscope, due to thepresence and radial arrangement of these hyaline bodies,gave rise to the name ray-fungus for this parasite. Notall of the granules have these clubs. In the granulesobtained from the lesions in man they are much less fre-quently observed than in those obtained from the lesions incattle. If a cover-glass preparation be made by breaking up oneof the granules and staining with Grams method, there willusually be found, upon examination with an oil-immersionlens, isolated and matted filaments, many of which may be. Fig. 108.—Branching actinomyces filaments in a cover-glass preparationmade from an actinomyces granule stained by Grams method; X 1000 (Wrightand Brown). seen to branch, in addition to longer and shorter fragmentsof filaments and fine detritus of the same (Fig. 108). Thefilaments are usually more or less wavy in their course, andare, as a rule, slightly thicker than the tubercle of the filaments will be found to stain homogeneously;others do not stain so deeply, and show numerous deeplystaining points in their substance. If clubs are present in PATHOGENIC BACTERIA AND FUNGI. 365 the granule, they also may be found scattered throughoutthe preparation. In sections of the tissues stained by Grams method twochief forms of granules are found. In one of these formsthe granule is seen to consist of filaments embedded in ahyaline substance, and usually arranged at the periphery


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Keywords: ., bookcentury1900, bookdecade1910, bookidpath, booksubjectpathology