. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ERYTHROPHORES OF PALAEMONETES 167 UJ O UJ §3 CL O tr cr LJ • o-o-o-o -SI >-OrO-O-O-O 75 150 0 MINUTES 75 150 FIGURE 1. Relationships between integumentary erythrophore stage and time in minutes. A: circles represent erythrophores in saline at outset; dots, erythrophores in colchicine (25 HIM) at outset. The pigment in these erythrophores was initially maximally dispersed. At the outset (at I) the erythrophores were placed in either colchicine or saline. Red pigment- concentrating hormone (RPCH) was administered to bot
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ERYTHROPHORES OF PALAEMONETES 167 UJ O UJ §3 CL O tr cr LJ • o-o-o-o -SI >-OrO-O-O-O 75 150 0 MINUTES 75 150 FIGURE 1. Relationships between integumentary erythrophore stage and time in minutes. A: circles represent erythrophores in saline at outset; dots, erythrophores in colchicine (25 HIM) at outset. The pigment in these erythrophores was initially maximally dispersed. At the outset (at I) the erythrophores were placed in either colchicine or saline. Red pigment- concentrating hormone (RPCH) was administered to both the saline-exposed and colchicine- treated erythrophores after 30 minutes (at II). The colchicine and RPCH were replaced by saline 60 minutes after 0 time (at III) and RPCH was administered again 90 minutes after 0 time (at IV) to the erythrophores that had been in the colchicine. B: all of the erythrophores (circles and dots) were exposed to colchicine (25 HIM) at the outset (at I). All received RPCH after 30 minutes (at II). Half of the erythrophore-containing pieces of carapace (dots) were placed in saline 60 minutes after 0 time (at III), and 90 minutes after 0 time (at IV) RPCH was again administered to the erythrophores that had been but were no longer in colchicine. piece of integument. When, according to the protocol, a hormone or other solu- tion was added to a depression containing an ovary or piece of integument and ml of saline or other solution, the additional volume was always ml. In every experiment either five experimental and five control ovaries or five experi- mental and five control pieces of integument were used. Each experiment was per- formed twice. Consequently, each data point in the following figures represents the mean of 10 determinations. EXPERIMENTS AND RESULTS Effect of 25 HIM colchicine on integumentary erythrophores The object of the first series of experiments was to determine the effects of 25 mM colchicine on pigment concentra
Size: 1005px × 2488px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
