. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HYBRID HORSESHOE CRAB LDH AND MDH 439 B. Tt tr rt rt Cr Cr Tg Tg Tg gt gt tg Tt Tt Tt 18 17 15 17 18 U U 18 19 19 20 (6) 19 20 U FIGURE 2. Electrophoretic patterns of the LDH in unfertilized eggs and developing embryos of 3 Asian horseshoe crabs and their hybrids. The hybridization experiments were carried out 2 times using different sets of 3 pairs (3 species) of horseshoe crabs. Electrophoretic patterns of the LDH in the 30th- day and 32nd-day embryos from the 2 experiments are shown separately in A and B, except for a col
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HYBRID HORSESHOE CRAB LDH AND MDH 439 B. Tt tr rt rt Cr Cr Tg Tg Tg gt gt tg Tt Tt Tt 18 17 15 17 18 U U 18 19 19 20 (6) 19 20 U FIGURE 2. Electrophoretic patterns of the LDH in unfertilized eggs and developing embryos of 3 Asian horseshoe crabs and their hybrids. The hybridization experiments were carried out 2 times using different sets of 3 pairs (3 species) of horseshoe crabs. Electrophoretic patterns of the LDH in the 30th- day and 32nd-day embryos from the 2 experiments are shown separately in A and B, except for a column Cr U in A. Horizontal starch gel electrophoresis was carried out at 4°C with 11% gel, using the same buffer system as explained in Figure 1. Cr, Tg, Tt, gt, rt, and tr are as described in Figure 1. tg = Hybrid between Tt 9 and Tg <5; U = unfertilized egg. Unfertilized eggs as well as fertilized eggs were cultured in sea water at 30°C for 3 days (A) and 32 days (B). Numbers indicate the developmental stage of Sekiguchi's normal plate (Sekiguchi, 1973). The number 6 in parentheses means that hybrid embryo used was able to live on until the 32nd day after insemination, although the development had stopped at stage 6 (blastula stage). 2B). Until this day the hybrid was able to live on, although the development had stopped at blastula stage, or stage 6 (Sekiguchi and Sugita, 1980; Sugita et al, 1982). The hybridized eggs of C. rotundicauda 9X7". gigas 6 and the reciprocal cross stopped their development at blastula stage (Sekiguchi and Sugita, 1980; Sugita et al., 1982) and never expressed the paternal forms of the LDH (results not shown). Malate dehydrogenase There are 2 major electrophoretic forms of the MDH in the horseshoe crab, Limulus polyphemus, as well as in most animals and higher plants. These isozymes are controlled by separate genetic loci and are localized in different subcellular fractions, a mitochondrial form and a supernatant form (Selander et al., 197
Size: 1419px × 1760px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
