. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 342 SHUHEI YUYAMA RESULTS Cleavage delay In the first series of experiments, Lytechinus eggs were inseminated and ex- posed to M ME for periods of 15 minutes, starting at various times after fertilization. Typical results are shown in Figure 1. During the first cleavage m in. LU o 10 CO CO LU 0 U X LU -10. 1 div. 2 div. T A I , mm. mm, o 0 LU A X^ i 20 RECOVERS D i i i i i 1 1 20 40 60 TIME in ME , m i n. FIGURE 2. Delay-ME exposure diagram of Arbacia eggs, (a) The experimental design is similar to that of Figure 1, alth
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 342 SHUHEI YUYAMA RESULTS Cleavage delay In the first series of experiments, Lytechinus eggs were inseminated and ex- posed to M ME for periods of 15 minutes, starting at various times after fertilization. Typical results are shown in Figure 1. During the first cleavage m in. LU o 10 CO CO LU 0 U X LU -10. 1 div. 2 div. T A I , mm. mm, o 0 LU A X^ i 20 RECOVERS D i i i i i 1 1 20 40 60 TIME in ME , m i n. FIGURE 2. Delay-ME exposure diagram of Arbacia eggs, (a) The experimental design is similar to that of Figure 1, although eggs are treated for 20 minutes in this experiment. Different lines indicate separate experiments. (i)) Dependence of the recovery period upon the duration of the ME treatment, the treatment was initiated either at the beginning of the streak stage (line 1) or several minutes before metaphase (line 2). cycle, there was a change in delay, although the exposure period was kept constant. With batches of cells whose first division cycle was 45 minutes, the delay decreased when the treatments were initiated up until 13 minutes after insemination, increased linearly up until 37 minutes and decreased rapidly after this time. During the period when delay increased linearly (the linear delay phase), the interval between the end of exposure and the time of cleavage was constant (Fig. 1). Although the period of the first cleavage cycle varied among different. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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