. Advances in herpetology and evolutionary biology : essays in honor of Ernest E. Williams. Williams, Ernest E. (Ernest Edward); Herpetology; Evolution. 622 Advances in Herpetology and Evolutionary Biology Webster et at., 1972; Hall and Selander, 1973; Paull et al, 1976). MATERIALS AND METHODS Specimens oiAnolis grahami were col- lected on Jamaica from the vicinity of Anchovy (N = 6, St. James Parish, A. g. grahami), Mandeville (N = 5, St. Thomas Parish, A. g. grahami), and Bath (N = 3, St. Thomas Parish, A. g. aquarum) during March 1980 (Fig. 1). All animals used in this study are deposited i
. Advances in herpetology and evolutionary biology : essays in honor of Ernest E. Williams. Williams, Ernest E. (Ernest Edward); Herpetology; Evolution. 622 Advances in Herpetology and Evolutionary Biology Webster et at., 1972; Hall and Selander, 1973; Paull et al, 1976). MATERIALS AND METHODS Specimens oiAnolis grahami were col- lected on Jamaica from the vicinity of Anchovy (N = 6, St. James Parish, A. g. grahami), Mandeville (N = 5, St. Thomas Parish, A. g. grahami), and Bath (N = 3, St. Thomas Parish, A. g. aquarum) during March 1980 (Fig. 1). All animals used in this study are deposited in the MCZ. One male from each population was C-banded (MCZ 158586, 158594, 158643). Chromosome samples were prepared in the field according to procedures de- scribed by Hall and Selander (1973). Animals were injected with colchicine 12 to 24 hours before dissection. Bone mar- row, testes, and spleen were removed and macerated in NaCitrate. Sus- pensions were centrifuged and hypotonic solution ( NaCitrate) added to each pellet, which was then suspended and held for 12 minutes before centrifuga- tion. After centrifugation (700 rpm for 7 minutes in an lEC tabletop centrifuge), fresh Camoy's fixative was added drop by drop to the pellet. This preparation can be stored in 2 mis of fixative at 4°C for at least 6 months. A drop of suspension in fresh fixative was deposited on an acid- cleansed slide which had been drained after being held in ice-cold 50% metha- nol. The slide was then tilted and air dried. Diploid number was determined from a minimum of 20 spreads in each animal. C-banding technique is modified from that of Sumner (1972) and Schmid (1978). Four- to seven-day-old slides are treated in HCl for 45 minutes and rinsed in distilled deionized water ( H2O). A series of slides is subjected to 5% Ba(OH)2 ^^ 37°C for times ranging from 1 to 5 minutes at one minute increments. After rinsing in H2O, the slides are incubated in 2XSSC (IX SSC is NaCl,
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Keywords: ., bookauthorharvarduniver, bookcentury1900, booksubjectherpetology
