. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. TISSUE CULTURE OF SILKWORM 179 TABLE I Pupal germ cells cultured in blood from larvae, prepupae, pupae, and developing adults Development in cultures Source of blood Age of donors (days) No. of donors No. of cultures None Slight Progres- sive Mature 5th instar larvae, not 0 3 15 14 1 0 yet spinning Larvae, spinning outer cap- 0-1 3 10 8 2* 0 sule of cocoon Larvae, spinning inner cap- 1-2 5 34 5 0 29 sule of cocoon Larvae, finished spinning 2-5 3 9 0 3* 6 cocoon Green prepupae 5-8 3 11 0 1* 10 White prepupae 8-9 2 10 0 4* 6 F
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. TISSUE CULTURE OF SILKWORM 179 TABLE I Pupal germ cells cultured in blood from larvae, prepupae, pupae, and developing adults Development in cultures Source of blood Age of donors (days) No. of donors No. of cultures None Slight Progres- sive Mature 5th instar larvae, not 0 3 15 14 1 0 yet spinning Larvae, spinning outer cap- 0-1 3 10 8 2* 0 sule of cocoon Larvae, spinning inner cap- 1-2 5 34 5 0 29 sule of cocoon Larvae, finished spinning 2-5 3 9 0 3* 6 cocoon Green prepupae 5-8 3 11 0 1* 10 White prepupae 8-9 2 10 0 4* 6 Fresh pupae 9-10 2 13 0 2* 11 Diapausing pupae After 3 weeks at 25° C. 4 17 2 9 6 Diapausing pupae After 5 to 16 weeks at 25 ° C. 13 40 26 14** 0 Chilled diapausing pupae After 12 to 35 weeks at 5° C. 7 23 20 3* • 0 Developing adults 1st to 5th day 15 47 0 6* 41 Developing adults 7th to 18th day 6 18 0 2* 16 * All cultures died within 48 hours after development was initiated. ** Six cultures died within 48 hours after development was Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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