. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 240 L. S. QUACKENBUSH AND M. FINGERMAN was purchased from Peninsula Laboratory (Belmont, California) and run on the Sephadex column as a standard. RESULTS Isolated eyes talks electrically stimulated Eyestalks stimulated with 10 volts or more released substances which induced significant black pigment dispersion in isolated crab legs (P < , WRS test) (Fig. 1). No significant red pigment dispersing activity was detected after voltage stimulation ( to 20 volts). Perfusates of eyestalks stimulated at 15 volts had signi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 240 L. S. QUACKENBUSH AND M. FINGERMAN was purchased from Peninsula Laboratory (Belmont, California) and run on the Sephadex column as a standard. RESULTS Isolated eyes talks electrically stimulated Eyestalks stimulated with 10 volts or more released substances which induced significant black pigment dispersion in isolated crab legs (P < , WRS test) (Fig. 1). No significant red pigment dispersing activity was detected after voltage stimulation ( to 20 volts). Perfusates of eyestalks stimulated at 15 volts had significantly more black pigment dispersing hormone (BPDH) activity than those given 10 volts (P < , WRS test). Black pigment concentrating hormone (BPCH) activity was detected in perfusates of eyestalks given volt; at 5 volts maximum BPCH activity was observed (P < , WRS test). RPCH activity was found in perfusates of eyestalks stimulated with volt (P < , WRS test). The apparently different thresholds for the release of BPDH and BPCH may have been due to the antagonistic actions of these two peptides on the target cells. Perfusates of eyestalks stimulated with 5 volts had no significant BPDH activity (Fig. 2). However, perfusates from eyestalks stimulated with 10 volts had significant BPDH activity and the BPCH activity was clearly antagonized by BPDH, as seen by the drop of BPCH activity in Figure 1, and Figure 2. RPCH activity was not antagonized by red pigment dispersing hormone (RPDH) in perfusates from eyestalks stimulated with 5 or 10 volts (Fig. 3). Either RPCH completely antagonizes the action of any RPDH that might have been released, or no RPDH was released with this stimulation. The data presented in Figure 1 demonstrate that the eyestalk releases several different factors when stimulated with 10 volts with this protocol. The isolated eyestalk may release only totipotent hormones which affect all chromatophores equally, as 25- 0- RPCH. 15 20
Size: 1692px × 1476px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
