. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FROG EGG PHOSPHOPROTEIN PHOSPHATASE 239 Figure 5 shows that the linear rate of phosphate release is only maintained as long as the rate of protein solubilization is linear. In addition, no activity of the extract against heated whole yolk in M NaCl was found although ghost-free YPA was solubilized and dephosphorylated under these conditions. These results suggest that disruption of the yolk platelet organization is essential for the action of the enzyme. Non-protein nitrogen did not increase during incubations with phosp
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FROG EGG PHOSPHOPROTEIN PHOSPHATASE 239 Figure 5 shows that the linear rate of phosphate release is only maintained as long as the rate of protein solubilization is linear. In addition, no activity of the extract against heated whole yolk in M NaCl was found although ghost-free YPA was solubilized and dephosphorylated under these conditions. These results suggest that disruption of the yolk platelet organization is essential for the action of the enzyme. Non-protein nitrogen did not increase during incubations with phosphoprotein, indicating that no proteolysis had taken place. 2. Enzyme concentration. The results summarized in Figure 6 show that a direct relationship exists between protein concentration and velocity with both phosphoprotein substrates tested. Variations in the salt concentration had little. 15 MINUTES 30 FIGURE 2. Rates of PPPase activity of centrifugal fractions of ovarian homogenates with or without added phosvitin. All incubations in pH buffer, M NaCl, 28° C. Filled circle: pigment + phosvitin. Circle, right half filled: small yolk + phosvitin. Filled triangle: whole homogenate + phosvitin. Circle, upper half filled: large yolk + phosvitin. Circle, lower half filled: large yolk endogenous activity. Open triangle: whole homogenate endogenous activity. Circle, left half filled: small yolk endogenous activity. Inset compares pigment and small yolk activities against phosvitin throughout the 30-minute Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole,
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