. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 5nA Figure 2. /Na in a muscle fiber from squid mantle. Skinned mantles from juvenile squid (55-57 days old) were cut into strips parallel to circular muscle bands in Ca-free seawater and treated with protease (Type XIV, Sigma) for 40 min at I 7°C. Strips were triturated through a micropipette to dissociate muscle fibers, which were then maintained up to one day at 17°C in a low-Ca medium (adapted from (15), external Ca = 2-3 mA/). (A) Video prints during a whole-cell recording (see text for details; scale bar for this and al
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 5nA Figure 2. /Na in a muscle fiber from squid mantle. Skinned mantles from juvenile squid (55-57 days old) were cut into strips parallel to circular muscle bands in Ca-free seawater and treated with protease (Type XIV, Sigma) for 40 min at I 7°C. Strips were triturated through a micropipette to dissociate muscle fibers, which were then maintained up to one day at 17°C in a low-Ca medium (adapted from (15), external Ca = 2-3 mA/). (A) Video prints during a whole-cell recording (see text for details; scale bar for this and all video prints = 20 pm). (B,) Inward current at 0 mV (holding potential = -80 mV for this and all other figures) from the fiber in panel A without (large amplitude trace) and with either a depolarizing prepulse (+pre) or exposure to 500 tetro- dotoxin (+TTX) in the external solution. (B,,) Records obtained in a GFL neuron (6 days in culture) using the same apparatus and under identical conditions as used for panel B,. (C) A family of/Nj currents (obtained by subtraction of records taken with and without an inacti- vating prepulse) recorded at the indicated voltages from the muscle fi- ber of panel A. External solution was culture medium. T= 10— I2° Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
