. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 550 B. R. RIVEST. ../â bw'v Figure 6. Sperm not injected into the gonad in Palio are phagocytosed by blood cells. This paraffin section of P. zosterae shows a mass of sperm that was injected into a peripheral interstice of the ovotestis and is now surrounded by blood cells, beâblood cells, bwâbody wall, hâhaemocoel, sâsperm. Bar = 100 Mm. Figure 7. Paraffin section through the visceral mass oiPalio zosterae showing the pathway by which a penial cirrus penetrated the body wall (large arrowhead) and gonad (small arrowhead). Th


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 550 B. R. RIVEST. ../â bw'v Figure 6. Sperm not injected into the gonad in Palio are phagocytosed by blood cells. This paraffin section of P. zosterae shows a mass of sperm that was injected into a peripheral interstice of the ovotestis and is now surrounded by blood cells, beâblood cells, bwâbody wall, hâhaemocoel, sâsperm. Bar = 100 Mm. Figure 7. Paraffin section through the visceral mass oiPalio zosterae showing the pathway by which a penial cirrus penetrated the body wall (large arrowhead) and gonad (small arrowhead). This section shows two lobes of the same injected spermatogenic acinus filled with unoriented exogenous sperm. The broken, outwardly displaced section of the epidermis is an artifact, bwâbody wall, enâendogenous sperm or spermatids, exâexogenous sperm, hâhaemocoel, hdâbranch of the hermaphroditic duct, iâinjected spermatogenic acinus, uâuninjected spermatogenic acinus. Bar = 100 ^m. mass, and ovotestis. Sperm were mixed in with the prostatic secretions in animals that were fixed immediately after copulation. In other animals, injected secretions not within the gonad were without sperm, and were found concentrated in body spaces as if they had settled there due to gravity or due to the circulation of the blood. Prostatic secretions injected into the ovotestis appeared to be rapidly transported to the bursa copulatrix. Animals often had exogenous sperm still within spermatogenic acini and the hermaphroditic duct when the only hint of prostatic secretions within the reproductive system was eosinophilic globules within the bursa copulatrix. The eight specimens of P. zosterae reared to maturity in isolation never laid egg masses while isolated. When placed together, they copulated by hypodermic injection as described above. Most of these animals were fixed shortly after copulation, but the two animals that were kept together for two days each laid a fertile egg mass. They


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