. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. — B Figure 1. Cross sections through an adult Eupnninu \cnlopes light organ. (A) Richardson's stained section: b. the inner bacteria-containing epithelium; d, the ciliated duct with its surrounding epithelium (duct does not reach the pore in this section): r. the reflector, i. the ink sac; and L. the light organ lens. Bar = 50 Mm. (B) Silver-enhanced immune section, counter-stained with acid fuchsin. The silver granules indicate the presence of L-crystallin in the light organ lens (dense black) and of an ALDH-like protein in
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. — B Figure 1. Cross sections through an adult Eupnninu \cnlopes light organ. (A) Richardson's stained section: b. the inner bacteria-containing epithelium; d, the ciliated duct with its surrounding epithelium (duct does not reach the pore in this section): r. the reflector, i. the ink sac; and L. the light organ lens. Bar = 50 Mm. (B) Silver-enhanced immune section, counter-stained with acid fuchsin. The silver granules indicate the presence of L-crystallin in the light organ lens (dense black) and of an ALDH-like protein in the ciliated duct epithelium (gray). The ink in the ink sac appears brown. (C) A pre-immune section lacks any dense black stain in the lens or duct epithelium. alkaline phosphatase immune blot kit (BioRad), which was designed to detect as little as 10 pg of antigen. The membrane was incubated in a 1:1000 dilution of the 1° antiserum for 2 h and subsequently incubated and de- veloped according to the manufacturer's directions. Results Localization ofALDII-like protein in adult light organs To determine the location of ALDH-like protein in the adult E. sco/opes light organ, immunocytochemistry, using anti-L-crystallin, was performed on cross sections of light organs at both the light microscopic and electron micro- scopic levels. In the 1 /jm thick sections, L-crystallin was localized in very high concentrations in the lens of the light organ as evidenced by the dense black silver nitrate granules visible against the pink acid fuchsin background stain (Fig. Ib, see Fig. la for orientation). No such black precipitate was present in the pre-immune incubation (Fig. Ic). Residual ink, present in the ink sac, appeared brown in the immune and pre-immune sections. Although not as dramatic as the staining in the lens, silver nitrate gran- ules were also present in the epithelial cells of the ciliated duct in the immune (Fig. Ib) but not in the pre-immune (Fig. Ic) preparations. In so
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
