. Cytology. Cytology. Focal plane of eyepiece Rear focal plane of objective Objective Microscope stage Condenser Figure 11-12. Diagram Illustrating the Optical Path of Deviated and Undeviated Light Through the Ordinary Light Microscope. transmitted by the object will tend to cancel each other out (destructive interference) and make the object darker than its surroundings (Figure 11-13). This is dark or positive phase-contrast (Figure 11-14 (a)). Bright or negative phase-contrast, in which the object appears brighter than its surroundings, is obtained by retarding the undeviated light by Va wav
. Cytology. Cytology. Focal plane of eyepiece Rear focal plane of objective Objective Microscope stage Condenser Figure 11-12. Diagram Illustrating the Optical Path of Deviated and Undeviated Light Through the Ordinary Light Microscope. transmitted by the object will tend to cancel each other out (destructive interference) and make the object darker than its surroundings (Figure 11-13). This is dark or positive phase-contrast (Figure 11-14 (a)). Bright or negative phase-contrast, in which the object appears brighter than its surroundings, is obtained by retarding the undeviated light by Va wavelength at the diffraction plate (conjugate area). Since the deviated light has already been retarded by V4 wavelength at the object, the 230 / CHAPTER 11. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Wilson, G. B. (George Bernard), 1914-; Morrison, John H. (John Herbert), 1927-. New York, Reinhold
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