. Contributions from the Laboratory of Plant Genetics. 254 GENETICS: D. KOSTOFF Proc. N. A. in about seven days, at which time the flowers usually fell. Acceleration as growth progressed was slight. Growth curves of pollen tubes after compatible matings, on the other hand, showed remarkable acceleration, and fertilization ensued after about three days. Since the genetic basis of this self- and cross-incompatibility in Nico- tiana is a series of multiple al- lelomorphs (East and Mangelsdorf, 1925, 1926), and since this interpre- tation does not appear to fit the phe- nomenon as exhibited in
. Contributions from the Laboratory of Plant Genetics. 254 GENETICS: D. KOSTOFF Proc. N. A. in about seven days, at which time the flowers usually fell. Acceleration as growth progressed was slight. Growth curves of pollen tubes after compatible matings, on the other hand, showed remarkable acceleration, and fertilization ensued after about three days. Since the genetic basis of this self- and cross-incompatibility in Nico- tiana is a series of multiple al- lelomorphs (East and Mangelsdorf, 1925, 1926), and since this interpre- tation does not appear to fit the phe- nomenon as exhibited in Lythrum salicaria—upon which an extended series of investigations has been carried out in this laboratory—it seemed desirable to determine pol- len-tube growth curves for "legiti- mate" and "illegitimate" unions in this species. Lythrum salicaria, it will be re- called, is a trimorphic self-sterile species which shows complete fer- tility only in unions where a given style is pollinated with pollen from stamens of the same tier. Other unions, the "illegitimate" unions, are comparatively infertile, though there is considerable variation among the twelve types of "illegitimate" union. The material, which consisted of a series of ovaries from a single mid- styled plant pollinated both "legitimately" and "illegitimately" with pollen from a single, long-styled plant/was given to me by Dr. E. M. East. It had been fixed partly in Flemming's medium solution and partly in Bourn s solution and imbedded in paraffin. The styles were fixed separately 3 hours, 6 hours, 12 hours, 24 hours, 36 hours, 48 hours, 60 hours, 72 hours and 85 hours after pollination. Several styles were available for each stated time. From this material, longitudinal sections 12,u thick were made, and stained with saffranin, Heidenhain's hamatoxilin or saffranin-gentian violet-orange G. The best preparations were those fixed in Flemming's solution and s
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