. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1000 600 Irequencylcm'l 1. ISOO 1000 600 frequency Icm'll Figure 1. The infrared ab^o^ption spectra from (a) the extracted water-insoluble organic matrix fraction of the Biomphalaha glabram shell; (b) the alkali-treated, protein-free water-insoluble organic matrix; (c) chitin from crabshell; (d) the extracted water-soluble organic matrix fraction of the B. glabnmi shell; (e) mucin from bovine submaxillary glands; (f) chondroitin sulfate A from bovine trachea. dase complex (Vector) was incubated for 30 min at room temperature
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1000 600 Irequencylcm'l 1. ISOO 1000 600 frequency Icm'll Figure 1. The infrared ab^o^ption spectra from (a) the extracted water-insoluble organic matrix fraction of the Biomphalaha glabram shell; (b) the alkali-treated, protein-free water-insoluble organic matrix; (c) chitin from crabshell; (d) the extracted water-soluble organic matrix fraction of the B. glabnmi shell; (e) mucin from bovine submaxillary glands; (f) chondroitin sulfate A from bovine trachea. dase complex (Vector) was incubated for 30 min at room temperature. After rinsing with PBS. the staining was carried out with (w/v) DAB, (w/v) and (v/v) HjO. in Tris-buffered solution, pH , for 20 min at room temperature. Controls: (1) without lectin, (2) without ABC, (3) without DAB, (4) lectins preincubated with their specific sugar. Results Hexosamine quantification and infrared spectrometry In this preparation, the IM of B. glabrata included the periostracum. Of the hydrolyzable part of the IM ( [w/w]), (w/w) was composed of hexosamines. After a previous alkaline treatment, alkali-resistant hexosa- mines represented (w/w) of the IM—that is, 85% (w/w) of the total hexosamines. The hexosamines of the SM were not alkali-resistant. The finding of alkali-resistant hexosamines in the IM hinted at the occurrence of chitin. The infrared absorption spectra of the IM and SM of the Bioniphalaria glahrata shell (Fig. 1) were examined to see whether chitin was visible in the IM and whether the occurrence of GAGs in the SM could be confirmed. The main absorption bands are listed in Table I. The pattern of the IM (Fig. la) differed from that of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass
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