Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . Method.—To 10 of defibri-nated blood add acetone until no more precipitate off the precipitated proteid and extract it with 10 ofacetone made acid with 2-3 drops of hydrochloric acid. Placea drop of the resulting colored extract on a slide, immediatelyplace a cover glass in position and examine under the micro-scope. Upon the evaporation of the acetone, crystals of haeminwill form. Larger crystals may be obtained by evaporati
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . Method.—To 10 of defibri-nated blood add acetone until no more precipitate off the precipitated proteid and extract it with 10 ofacetone made acid with 2-3 drops of hydrochloric acid. Placea drop of the resulting colored extract on a slide, immediatelyplace a cover glass in position and examine under the micro-scope. Upon the evaporation of the acetone, crystals of haeminwill form. Larger crystals may be obtained by evaporatingthe acetone extract about one-half, transferring it to a stop-pered vessel and allowing it to remain over night. (c) Schalfijews Method.—Place 20 of glacial acetic 1 Buckmaster advises the use of an alcoholic solution of guaiaconicacid instead of an alcoholic solution of guaiac Buckmaster considers the use of potassium chloride preferable. 164 PHYSIOLOGICAL CHEMISTRY. Pic. 58. Hjemin Crystals from Human Blood. Reproduced from a micro-photograph furnished by Prof. E. T. Reichert, of the University of Pennsylvania. Fig. H;emin Crystals from Sheep Blood. Reproduced from a micro-photograph furnished by Prof. E. T. Reichert, of the University of Pennsylvania. BLOOD. 165 acid in a small beaker and heat to 8oc C. Add 5 ofstrained defibrinated blood, again bring the temperature to80 C, remove the flame and allow the mixture* to the crystals under the microscope and compare themwith those reproduced in Figs. 58 and 59, page 1^4. 12. Catalytic Action.—To about 10 drops of blood in atest-tube add twice the volume of hydrogen peroxide, withoutshaking. The mixture foams. What is the cause of thisphenomenon ? [3. Preparation of Haematin.—Place 100 of lakedbli k m1 in a beaker and add 95 per cent alcohol until precipitationceases. What bodies are precipitated? Transfer the precipi-tate to a flask and boil with 95 per cent alcohol previouslyac
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