. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CALCITONIN FROM CARTILAGINOUS RAY 487 I o CO CM 60 50 t m 40 o 30 20 40 60 Time (mm) Figure 3. Reverse phase HPLC on an ODS-120T column. Sample, fraction 12 in Figure 2; flow rate. 1 ml/min. Solvent system: linear- gradient elution from solvent A (H:O : CH,CN : 1 M NH4OAc, pH = 72 : 8 : 1. v/v) to B (H,O : CH3CN : 1 M NH4OAc. pH = 25 : 100 : 1. v/v) for 40 min. Fraction was collected at each peak. Immunoreactivity appeared only in the peak marked by the arrow. 32 amino acid residues: Asp, (2); Glu
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CALCITONIN FROM CARTILAGINOUS RAY 487 I o CO CM 60 50 t m 40 o 30 20 40 60 Time (mm) Figure 3. Reverse phase HPLC on an ODS-120T column. Sample, fraction 12 in Figure 2; flow rate. 1 ml/min. Solvent system: linear- gradient elution from solvent A (H:O : CH,CN : 1 M NH4OAc, pH = 72 : 8 : 1. v/v) to B (H,O : CH3CN : 1 M NH4OAc. pH = 25 : 100 : 1. v/v) for 40 min. Fraction was collected at each peak. Immunoreactivity appeared only in the peak marked by the arrow. 32 amino acid residues: Asp, (2); Glu, (4); CM- Cys, (2); Ser, (3); Gly, (2); His, (1); Arg, l.'l (l);Thr, (4); Ala, (2); Pro, (1), Val. (3). He. (1), Leu. (4), Lys, (2); the numbers in pa- rentheses were deduced from the sequencing. Since the cysteine residue was undetectable without carboxyme- th\ lation in a sequencer, the carboxymethylated material was also subjected to the sequencer and the presence of cysteine residues at the first and the seventh position was confirmed. The amidation of the proline residue at the C-terminus was determined by co-chromatography with a synthetic peptide in reverse-phase HPLC. Discussion In this study, a large amount of calcitonin (more than 60 nmoles/g tissue) has been detected in the ultimobran- chial glands of the ray (Dasyatis akajei), and sequenced. This is the first elasmobranch calcitonin to have been characterized. In mammals, calcitonin is a hypocalcemic hormone that inhibits the reabsorption of calcium from the bone (Friedman and Raisz, 1965). Among non-mammals, cal- citonin-like immunoreactivity has been detected in se- lected species from all classes, and calcitonin has been isolated in a bird and teleosts (Niall et al., 1969; Van Noorden and Pearse, 1971; Noda and Narita, 1976; Tis- serand-Jochem et a!., 1977; Sasayama el al,, 1984; Treil- hou-Lahille et al., 1984; Homma et al., 1986), but the physiological roles of the
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
