Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . ich of courseshould be subtracted from thetotal amount of ammonia asdetermined by the above Folin method is extremelyaccurate under all conditionscreept when the urine containssugar. When this is the casethe carbohydrate and the ureaunite, upon being heated, andform a very stable this reason the Folin methodis not suitable for use in theexamination of such best method for use undersuch conditions is the combi-
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . ich of courseshould be subtracted from thetotal amount of ammonia asdetermined by the above Folin method is extremelyaccurate under all conditionscreept when the urine containssugar. When this is the casethe carbohydrate and the ureaunite, upon being heated, andform a very stable this reason the Folin methodis not suitable for use in theexamination of such best method for use undersuch conditions is the combi-nation M6rner-Sjoqvist-F o 1 i nmethod which is given Morner - Sjoqvist - FolinMethod.—As has already beenstated in the last experimentthis method excels the Folinmethod in accuracy only in thedetermination of urea in thepresence of carbohydrate bodies. Briefly the procedure is asfollows:1 Bring the major portion of grams of powderedbarium hydroxide into solution in 5 of urine in a small 1 The original description of the method may be found in an article byMorner: Skandinavisches Archiv fur Physiologic, 1903, xiv, p. Folins Urea Apparatus. urine: quantitative analysis. 357 flask, and treat the mixture with of an alcohol-ether so-lution, consisting of two volumes of 97 per cent alcohol and onevolume of ether. Stopper the flask and allow it to stand 12-24hours. Filter off the precipitate, wash it with the alcohol-ethermixture and remove the alcohol and ether from the filtrate bydistillation, being careful to keep the temperature of the mix-ture below 50 Treat the remaining fluid (about 25 )with 2 of hydrochloric acid i sp. gr. [.124) transfer it care-fully to a 200 flask and evaporate the mixture to dryness ona water-bath. Now add 20 grams of crystallized magnesiumchloride and 2 of concentrated hydrochloric acid to theresidue and after fitting the flask with a return cooler boil themixture on a wire gauze over a small flame
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