. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALL STAGES (HYDROLYZED). o PHENOL FIGURE 1. Diagram of the results of two-dimensional chromatography on hydrolyzed tissue of D. discoideum. Spots are identified as the leucines (1), phenylalanine (2), methionine (3), proline (4), tyrosine (5), alanine (6), threonine (7), histidine (8), glycine (9), glutamic acid (10), serine (11), asparagine (12), unknown (13), cystine (14), and aspartic acid (15). no more than five minutes in any case. Rupture of virtually all cells was insured by means of periodic microscopic examination o
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALL STAGES (HYDROLYZED). o PHENOL FIGURE 1. Diagram of the results of two-dimensional chromatography on hydrolyzed tissue of D. discoideum. Spots are identified as the leucines (1), phenylalanine (2), methionine (3), proline (4), tyrosine (5), alanine (6), threonine (7), histidine (8), glycine (9), glutamic acid (10), serine (11), asparagine (12), unknown (13), cystine (14), and aspartic acid (15). no more than five minutes in any case. Rupture of virtually all cells was insured by means of periodic microscopic examination of the homogenate. For both types of analyses, , hydrolyzed and unhydrolyzed, two-dimensional chromatograms were made on Whatman No. 1 filter paper. For the first dimension, n-butanol, acetic acid and water (250, 60, 250 v/v/v) were used as the solvent mixture. For the second dimension, an 80% solution of phenol in water was used as solvent. Development of the spots was accomplished by means of spraying the chromatograms with a solution of ninhydrin in 95% ethanol. After. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
